Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Thomsen, M.; Tuukkanen, A.; Dickerhoff, J.; Palm, G.J.; Kratzat, H.; Svergun, D.I.; Weisz, K.; Bornscheuer, U.T.; Hinrichs, W.
    Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase (2015), Acta Crystallogr. Sect. D, 71, 907-917 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.6 expressed in Escherichia coli Rosetta (DE3) cells Eubacterium ramulus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.6 in complex with (2S)-naringenin, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 6% (v/v) ethanol, 0.37 mM naringenin chalcone Eubacterium ramulus

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.6 H33A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
5.5.1.6 H33E the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
5.5.1.6 H33Q the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
5.5.1.6 H73A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
5.5.1.6 R125A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Eubacterium ramulus
5.5.1.6 Y48F the mutant shows increased catalytic efficiency compared to the wild type enzyme Eubacterium ramulus

General Stability

EC Number General Stability Organism
5.5.1.6 the activity is not affected by DMSO concentrations of up to 5% (v/v) Eubacterium ramulus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.6 0.0143
-
naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.0203
-
naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.0439
-
naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.0567
-
naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.0798
-
naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.1319
-
naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.354
-
naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.6 194000
-
gel filtration Eubacterium ramulus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.6 (2S)-naringenin Eubacterium ramulus
-
naringenin chalcone
-
r
5.5.1.6 naringenin chalcone Eubacterium ramulus
-
(2S)-naringenin
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.6 Eubacterium ramulus V9P0A9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.6 ammonium sulfate precipitation, DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and Superdex 200 gel filtration Eubacterium ramulus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.6 (2S)-naringenin
-
Eubacterium ramulus naringenin chalcone
-
r
5.5.1.6 naringenin chalcone
-
Eubacterium ramulus (2S)-naringenin
-
r

Subunits

EC Number Subunits Comment Organism
5.5.1.6 homohexamer 6 * 32400, calculated from amino acid sequence Eubacterium ramulus

Synonyms

EC Number Synonyms Comment Organism
5.5.1.6 CHI
-
Eubacterium ramulus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.6 0.07
-
naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.2
-
naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 0.6
-
naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 784
-
naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 1142
-
naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 1313
-
naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 1379
-
naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.5.1.6 0.9
-
naringenin chalcone mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 1.6
-
naringenin chalcone mutant enzyme H33E, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 29
-
naringenin chalcone mutant enzyme H33A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 24000
-
naringenin chalcone mutant enzyme R125A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 28000
-
naringenin chalcone mutant enzyme H73A, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 32000
-
naringenin chalcone wild type enzyme, at pH 6.4, temperature not specified in the publication Eubacterium ramulus
5.5.1.6 55000
-
naringenin chalcone mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication Eubacterium ramulus