EC Number | Cloned (Comment) | Organism |
---|---|---|
5.5.1.6 | expressed in Escherichia coli Rosetta (DE3) cells | Eubacterium ramulus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.5.1.6 | in complex with (2S)-naringenin, hanging drop vapor diffusion method, using 2.0 M ammonium sulfate, 0.2 M NaCl, 0.1 M HEPES pH 7.5, 6% (v/v) ethanol, 0.37 mM naringenin chalcone | Eubacterium ramulus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.5.1.6 | H33A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
5.5.1.6 | H33E | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
5.5.1.6 | H33Q | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
5.5.1.6 | H73A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
5.5.1.6 | R125A | the mutant shows reduced catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
5.5.1.6 | Y48F | the mutant shows increased catalytic efficiency compared to the wild type enzyme | Eubacterium ramulus |
EC Number | General Stability | Organism |
---|---|---|
5.5.1.6 | the activity is not affected by DMSO concentrations of up to 5% (v/v) | Eubacterium ramulus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.6 | 0.0143 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.0203 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.0439 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.0567 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.0798 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.1319 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.354 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.5.1.6 | 194000 | - |
gel filtration | Eubacterium ramulus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.6 | (2S)-naringenin | Eubacterium ramulus | - |
naringenin chalcone | - |
r | |
5.5.1.6 | naringenin chalcone | Eubacterium ramulus | - |
(2S)-naringenin | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.5.1.6 | Eubacterium ramulus | V9P0A9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.6 | ammonium sulfate precipitation, DEAE-Sepharose column chromatography, butyl-Sepharose column chromatography, and Superdex 200 gel filtration | Eubacterium ramulus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.6 | (2S)-naringenin | - |
Eubacterium ramulus | naringenin chalcone | - |
r | |
5.5.1.6 | naringenin chalcone | - |
Eubacterium ramulus | (2S)-naringenin | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.5.1.6 | homohexamer | 6 * 32400, calculated from amino acid sequence | Eubacterium ramulus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.5.1.6 | CHI | - |
Eubacterium ramulus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.6 | 0.07 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.2 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 0.6 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 784 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 1142 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 1313 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 1379 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.5.1.6 | 0.9 | - |
naringenin chalcone | mutant enzyme H33Q, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 1.6 | - |
naringenin chalcone | mutant enzyme H33E, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 29 | - |
naringenin chalcone | mutant enzyme H33A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 24000 | - |
naringenin chalcone | mutant enzyme R125A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 28000 | - |
naringenin chalcone | mutant enzyme H73A, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 32000 | - |
naringenin chalcone | wild type enzyme, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus | |
5.5.1.6 | 55000 | - |
naringenin chalcone | mutant enzyme Y48F, at pH 6.4, temperature not specified in the publication | Eubacterium ramulus |