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High-resolution structures of mutants of residues that affect access to the ligand-binding cavity of human lipocalin-type prostaglandin D synthase

Perduca, M.; Bovi, M.; Bertinelli, M.; Bertini, E.; Destefanis, L.; Carrizo, M.E.; Capaldi, S.; Monaco, H.L.; Acta Crystallogr. Sect. D 70, 2125-2138 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.2
mutant enzymes C65A/K59A, C65A, C65A/W54F, C65A/W112F and C65A/W54F/W112F hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 30% (w/v) PEG 4000
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Homo sapiens
-
Prostaglandin D2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Homo sapiens
P41222
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
chitin column chromatography and Superdex G-75 gel filtration
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
746616
Homo sapiens
Prostaglandin D2
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.2
mutant enzymes C65A/K59A, C65A, C65A/W54F, C65A/W112F and C65A/W54F/W112F hanging drop vapor diffusion method, using 0.2 M ammonium sulfate, 30% (w/v) PEG 4000
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Homo sapiens
-
Prostaglandin D2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
chitin column chromatography and Superdex G-75 gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
Prostaglandin H2
-
746616
Homo sapiens
Prostaglandin D2
-
-
-
?