EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.203 | N-bromosuccinimide | 114.27% activity at 10 mM | Bacillus velezensis | |
3.2.1.203 | TPCK | 121.32% activity at 10 mM | Bacillus velezensis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.203 | iodoacetic acid | 30.33% residual activity at 10 mM | Bacillus velezensis | |
3.2.1.203 | phenyl glyoxylate | 75.06% residual activity at 10 mM | Bacillus velezensis | |
3.2.1.203 | phenylmethylsulfonyl fluoride | 35.54% residual activity at 10 mM | Bacillus velezensis | |
3.2.1.203 | TLCK | 72.15% residual activity at 10 mM | Bacillus velezensis | |
3.2.1.203 | Woodward's reagent K | 56.98% residual activity at 10 mM | Bacillus velezensis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.203 | Ca2+ | 114.2% activity at 100 mM | Bacillus velezensis | |
3.2.1.203 | Cd2+ | 114.2% activity at 100 mM | Bacillus velezensis | |
3.2.1.203 | Co2+ | 319.1% activity at 100 mM | Bacillus velezensis | |
3.2.1.203 | K+ | 148.4% activity at 100 mM | Bacillus velezensis | |
3.2.1.203 | Mg2+ | 141.2% activity at 100 mM | Bacillus velezensis | |
3.2.1.203 | Na+ | 103.1% activity at 100 mM | Bacillus velezensis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.203 | Bacillus velezensis | - |
- |
- |
3.2.1.203 | Bacillus velezensis Y37 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.203 | phenyl Sepharose column chromatography | Bacillus velezensis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.203 | 10.39 | - |
using (carboxymethyl)cellulose as substrate, at pH 7.0 and 50°C | Bacillus velezensis |
3.2.1.203 | 13.13 | - |
using filter paper as substrate, at pH 7.0 and 50°C | Bacillus velezensis |
3.2.1.203 | 14.19 | - |
using laminarin as substrate, at pH 7.0 and 50°C | Bacillus velezensis |
3.2.1.203 | 14.89 | - |
using glucan as substrate, at pH 7.0 and 50°C | Bacillus velezensis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.203 | (carboxymethyl)cellulose + H2O | - |
Bacillus velezensis | ? | - |
? | |
3.2.1.203 | (carboxymethyl)cellulose + H2O | - |
Bacillus velezensis Y37 | ? | - |
? | |
3.2.1.203 | filter paper + H2O | - |
Bacillus velezensis | ? | - |
? | |
3.2.1.203 | filter paper + H2O | - |
Bacillus velezensis Y37 | ? | - |
? | |
3.2.1.203 | glucan + H2O | highest activity | Bacillus velezensis | ? | - |
? | |
3.2.1.203 | glucan + H2O | highest activity | Bacillus velezensis Y37 | ? | - |
? | |
3.2.1.203 | laminarin + H2O | - |
Bacillus velezensis | ? | - |
? | |
3.2.1.203 | laminarin + H2O | - |
Bacillus velezensis Y37 | ? | - |
? | |
3.2.1.203 | additional information | no activity with 2-nitrophenyl beta -D-galactopyranoside | Bacillus velezensis | ? | - |
? | |
3.2.1.203 | additional information | no activity with 2-nitrophenyl beta -D-galactopyranoside | Bacillus velezensis Y37 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.203 | ? | x * 50000, SDS-PAGE | Bacillus velezensis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.203 | CMCase | - |
Bacillus velezensis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.203 | 45 | - |
- |
Bacillus velezensis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.203 | 55 | 65 | more than 90% of the original enzyme activity is maintained at temperatures ranging from 55 to 65°C after 45 min. Above 65°C, the enzyme activity is moderately lost with 65% remaining | Bacillus velezensis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.203 | 5 | - |
- |
Bacillus velezensis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.203 | 10 | - |
after incubation at 4°C and pH 10 for 3 h the enzyme retains 82% of the original activity, while this is 74% for incubation at 50°C and pH 10 for 1 h | Bacillus velezensis |