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Literature summary extracted from

  • Montoya-Rosales, A.; Provvedi, R.; Torres-Juarez, F.; Enciso-Moreno, J.; Hernandez-Pando, R.; Manganelli, R.; Rivas-Santiago, B.
    lysX gene is differentially expressed among Mycobacterium tuberculosis strains with different levels of virulence (2017), Tuberculosis, 106, 106-117 .
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.43 medicine in the presence of antimicrobial peptides produced by epithelial cells and macrophages, lysX expression increases significantly. Strains with higher lysX expression show increased levels of intracellular survival in vivo and in vitro and induce more severe lesion related with pneumonia. The ability of Mycobacterium tuberculosis to replicate intracellularly is directly correlated to the level of lysX expression Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.3 gene lysX, conditional expression of lysX in Mycobacterium tuberculosis strain ATCC 25618 / H37Rv ia achieved by an anhydrotetracycline (ATc)-inducible expression system. Gene lysX expression is related to Mycobacterium tuberculosis virulence Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol Mycobacterium tuberculosis
-
 tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol Mycobacterium tuberculosis H37Rv
-
 tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol Mycobacterium tuberculosis ATCC 25618
-
 tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.3 Mycobacterium tuberculosis P9WFU7 several clinical isolates
-
2.3.2.3 Mycobacterium tuberculosis ATCC 25618 P9WFU7 several clinical isolates
-
2.3.2.3 Mycobacterium tuberculosis H37Rv P9WFU7 several clinical isolates
-
6.3.2.43 Mycobacterium tuberculosis
-
-
-
6.3.2.43 Mycobacterium tuberculosis A5U2Z7
-
-
6.3.2.43 Mycobacterium tuberculosis P9WFU7
-
-
6.3.2.43 Mycobacterium tuberculosis H37Rv P9WFU7
-
-
6.3.2.43 Mycobacterium tuberculosis LAM09005186
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.3 additional information the lysX gene is differentially expressed among Mycobacterium tuberculosis strains 37 (strains H37Rv, H37Ra, Beijing, Beijing4P, MDR, and 5186) Mycobacterium tuberculosis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol
-
 Mycobacterium tuberculosis tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol
-
 Mycobacterium tuberculosis H37Rv tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?
2.3.2.3 L-lysyl-tRNALys + phosphatidylglycerol
-
 Mycobacterium tuberculosis ATCC 25618 tRNALys + 3-O-L-lysyl-1-O-phosphatidylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.2.3 LysX
-
 Mycobacterium tuberculosis
2.3.2.3 Rv1640c
-
 Mycobacterium tuberculosis
6.3.2.43 LysX
-
 Mycobacterium tuberculosis

Expression

EC Number Organism Comment Expression
2.3.2.3 Mycobacterium tuberculosis in the presence of AMPs, lysX expression increases significantly. Strains with higher lysX expression show increased levels of intracellular survival in vivo and in vitro and induce more severe lesion related with pneumonia. Comparisons of lysX absolute expression in different strains of Mycobacterium tuberculosis with and without LL-37 (strains H37Rv, H37Ra, Beijing, Beijing4P, MDR, and 5186). Strains H37Rv and Beijing strains similar results are obtained after 30 and 60 min of incubation. H37Ra and Beijing strains do not show differences among stimulation times whereas Beijing, Beijing4P, MDR and 5186 strains showed statistic differences among stimulation times when compared with the respective NoTx condition up
6.3.2.43 Mycobacterium tuberculosis in the presence of antimicrobial peptides, lysX expression increases significantly up

General Information

EC Number General Information Comment Organism
2.3.2.3 physiological function Mycobacterium tuberculosis (Mtb) has developed mechanisms to avoid antimicrobial peptides (AMPs) activity, for instance lysX adds lysine residues to surface phospholipids changing their net charge, leading to the repelling of the AMPs. In the presence of AMPs, lysX expression increases significantly. Strains with higher lysX expression show increased levels of intracellular survival in vivo and in vitro and induce more severe lesion related with pneumonia. Ability of Mtb to replicate intracellularly is directly correlated to the level of lysX expression showing that the amount of lysX produced by the bacterial cell is an important variable for the modulation of Mtb virulence. Gene expression of AMPs during in vivo infection of BALB/c mice, overview Mycobacterium tuberculosis
6.3.2.43 physiological function in the presence of antimicrobial peptides produced by epithelial cells and macrophages, lysX expression increases significantly. Strains with higher lysX expression show increased levels of intracellular survival in vivo and in vitro and induce more severe lesion related with pneumonia. The ability of Mycobacterium tuberculosis to replicate intracellularly is directly correlated to the level of lysX expression Mycobacterium tuberculosis