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Literature summary extracted from

  • Jain, V.; Yogavel, M.; Oshima, Y.; Kikuchi, H.; Touquet, B.; Hakimi, M.A.; Sharma, A.
    Structure of prolyl-tRNA synthetase-halofuginone complex provides basis for development of drugs against malaria and toxoplasmosis (2015), Structure, 23, 819-829 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.15 hanging drop vapor diffusion method, using 0.1 M Bis-Tris (pH 6.5), 2 % (v/v) Tacsimate (pH 5.0), and 12% (w/v) polyethylene glycol 3350 Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 6-fluoro-febrifugine
-
 Plasmodium falciparum
6.1.1.15 6-fluoro-febrifugine
-
 Toxoplasma gondii
6.1.1.15 7-bromo-6-chloro-3-[3-[3-hydroxy-2-piperidinyl]-2-oxopropyl]-4-quinazolinone
-
 Plasmodium falciparum
6.1.1.15 7-bromo-6-chloro-3-[3-[3-hydroxy-2-piperidinyl]-2-oxopropyl]-4-quinazolinone
-
 Toxoplasma gondii
6.1.1.15 febrifugine
-
 Plasmodium falciparum
6.1.1.15 febrifugine
-
 Toxoplasma gondii
6.1.1.15 halofuginone
-
 Plasmodium falciparum
6.1.1.15 halofuginone
-
 Toxoplasma gondii
6.1.1.15 tetrahydrofebrifugine
-
 Plasmodium falciparum
6.1.1.15 tetrahydrofebrifugine
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Toxoplasma gondii
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Plasmodium falciparum
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Plasmodium falciparum Q8I5R7
-
-
6.1.1.15 Toxoplasma gondii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 adenosine 5'-(beta,gamma-imido)triphosphate + L-proline + tRNAPro
-
 Toxoplasma gondii ?
-
 ?
6.1.1.15 adenosine 5'-(beta,gamma-imido)triphosphate + L-proline + tRNAPro
-
 Plasmodium falciparum ?
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Toxoplasma gondii AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Plasmodium falciparum AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Toxoplasma gondii
6.1.1.15 Prolyl-tRNA synthetase
-
 Plasmodium falciparum
6.1.1.15 PRS
-
 Toxoplasma gondii
6.1.1.15 PRS
-
 Plasmodium falciparum

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Toxoplasma gondii
6.1.1.15 ATP
-
 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.15 0.000009
-
 at pH 7.4 and 37°C Plasmodium falciparum halofuginone
6.1.1.15 0.000013
-
 at pH 7.4 and 37°C Plasmodium falciparum 6-fluoro-febrifugine
6.1.1.15 0.000024
-
 at pH 7.4 and 37°C Plasmodium falciparum febrifugine
6.1.1.15 0.000352
-
 at pH 7.4 and 37°C Plasmodium falciparum tetrahydrofebrifugine