EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.77 | gene anr, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis | Fagopyrum tataricum |
1.17.1.3 | gene lar, DNA and amino acid sequence determination and analysis, sequence comaprisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis | Fagopyrum tataricum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.77 | chloroplast | - |
Fagopyrum tataricum | 9507 | - |
1.3.1.77 | cytosol | - |
Fagopyrum tataricum | 5829 | - |
1.17.1.3 | cytosol | - |
Fagopyrum tataricum | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.77 | anthocyanidin + 2 NADPH + H+ | Fagopyrum tataricum | - |
epicatechin + 2 NADP+ | ? | ? | |
1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | Fagopyrum tataricum | - |
(2R,3S)-catechin + NADP+ + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.77 | Fagopyrum tataricum | V9PJG5 | cvs. Hokkai T8 and Hokkai T10 | - |
1.17.1.3 | Fagopyrum tataricum | V9PJ26 | cvs. Hokkai T8 and Hokkai T10 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.77 | flower | - |
Fagopyrum tataricum | - |
1.3.1.77 | hairy root | - |
Fagopyrum tataricum | - |
1.3.1.77 | leaf | - |
Fagopyrum tataricum | - |
1.3.1.77 | additional information | gene expression of FtLAR during sprout development under dark and light conditions in cvs. T8 and T10, determination of catechin and epicatechin contents in sprouts, the expression pattern of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) do not match the accumulation pattern of proanthocyanidins in different organs of the two cultivars Hokkai T8 and Hokkai T10. FtANR expression levels are higher in T8 than T10 under both conditions. Specifically, transcript level in T8 under dark condition is higher than that under light condition, although fluctuation pattern is observed in light condition. FtANR expression is increased from 0 to 3 days-after-sowing for T8 cultivar under dark condition and remained relatively constant on 6, 9, and 12 days-after-sowing. The catechin content is correlated with color pigment. Epicatechin content is affected by light in T8 only, whereas T10 remains unaffected by light. Flowers of T8 and T10 contain the highest levels of epicatechin, whereas the lowest amount of epicatechin is in the seeds at stage 3 in T8 | Fagopyrum tataricum | - |
1.3.1.77 | root | - |
Fagopyrum tataricum | - |
1.3.1.77 | seed | - |
Fagopyrum tataricum | - |
1.3.1.77 | seedling | - |
Fagopyrum tataricum | - |
1.3.1.77 | sprout | grown under light or dark condition | Fagopyrum tataricum | - |
1.3.1.77 | stem | - |
Fagopyrum tataricum | - |
1.17.1.3 | flower | - |
Fagopyrum tataricum | - |
1.17.1.3 | hairy root | - |
Fagopyrum tataricum | - |
1.17.1.3 | leaf | - |
Fagopyrum tataricum | - |
1.17.1.3 | additional information | gene expression of FtLAR during sprout development under dark and light conditions in cvs. T8 and T10, determination of catechin and epicatechin contents in sprouts, the expression pattern of anthocyanidin reductase (ANR) and leucoanthocyanidin reductase (LAR) do not match the accumulation pattern of proanthocyanidins in different organs of the two cultivars Hokkai T8 and Hokkai T10. FtLAR does not show large variations in its expression patterns among all the organs in cultivars Hokkai T8 and T10. Epicatechin content is higher than the catechin content in both T8 and T10. In T10, catechin is found mostly in the flowers and roots, whereas in T8, higher amounts are found in leaves and seeds at stage 1. Catechin content in T10 is highest in roots. Hokkai T10 wild roots in the soil and hairy roots are red in color. The catechin content is correlated with color pigment | Fagopyrum tataricum | - |
1.17.1.3 | root | - |
Fagopyrum tataricum | - |
1.17.1.3 | seed | - |
Fagopyrum tataricum | - |
1.17.1.3 | seedling | - |
Fagopyrum tataricum | - |
1.17.1.3 | sprout | grown under light or dark condition | Fagopyrum tataricum | - |
1.17.1.3 | stem | - |
Fagopyrum tataricum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.77 | anthocyanidin + 2 NADPH + H+ | - |
Fagopyrum tataricum | epicatechin + 2 NADP+ | ? | ? | |
1.17.1.3 | 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ | - |
Fagopyrum tataricum | (2R,3S)-catechin + NADP+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.77 | ? | x * 36800, about, sequence calculation | Fagopyrum tataricum |
1.17.1.3 | ? | x * 43300, about, sequence calculation | Fagopyrum tataricum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.77 | ANR | - |
Fagopyrum tataricum |
1.3.1.77 | anthocyanidin reductase | - |
Fagopyrum tataricum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.77 | NADPH | - |
Fagopyrum tataricum | |
1.17.1.3 | NADPH | - |
Fagopyrum tataricum |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.77 | Fagopyrum tataricum | sequence calculation | - |
5.42 |
1.17.1.3 | Fagopyrum tataricum | sequence calculation | - |
5.27 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.77 | metabolism | the enzyme is important in biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin, the proanthocyanidin pathway exists as ametabolic channel associated with cellular membranes | Fagopyrum tataricum |
1.3.1.77 | physiological function | anthocyanidin reductase (ANR), together with leucoanthocyanidin reductase (LAR, EC 1.17.1.3), plays an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. The catechin content is correlated with color pigment in roots | Fagopyrum tataricum |
1.17.1.3 | metabolism | the enzyme is important in biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin, the proanthocyanidin pathway exists as ametabolic channel associated with cellular membranes | Fagopyrum tataricum |
1.17.1.3 | additional information | FtLAR had specific amino acid motifs of ICCN and THD | Fagopyrum tataricum |
1.17.1.3 | physiological function | leucoanthocyanidin reductase (LAR), together with anthocyanidin reductase (ANR, EC 1.3.1.77), plays an important role in the monomeric units biosynthesis of proanthocyanidins (PAs) such as catechin and epicatechin in several plants. ANR and LAR levels in tartary buckwheat might be regulated by different mechanisms for catechin and epicatechin biosynthesis under light and dark conditions. The catechin content is correlated with color pigment in roots | Fagopyrum tataricum |