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Literature summary extracted from

  • Godoy, A.S.; Pereira, C.S.; Ramia, M.P.; Silveira, R.L.; Camilo, C.M.; Kadowaki, M.A.; Lange, L.; Busk, P.K.; Nascimento, A.S.; Skaf, M.S.; Polikarpov, I.
    Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members (2018), Sci. Rep., 8, 3678 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Aspergillus niger Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.4 to 1.5 A resolution. Comparison between enzyme and other representative GH45 members Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 D114A complete loss of activity Phanerodontia chrysosporium
3.2.1.4 D85A about 10% of wild-type activity Phanerodontia chrysosporium
3.2.1.4 N92D replaxement of the general base in the catalytic mechanism, drastic decrease in activity. Unlike Asn92, residue Asp92 is mobile Phanerodontia chrysosporium
3.2.1.4 W154A about 50% of wild-type activity Phanerodontia chrysosporium
3.2.1.4 Y18A about 80% of wild-type activity Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 carboxymethylcellulose Km value 2 mg/mlpH 4.0, 70°C Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Phanerodontia chrysosporium B3Y002
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethylcellulose + H2O
-
 Phanerodontia chrysosporium ?
-
 ?
3.2.1.4 additional information no substrates: avicel, arabinoxylan and xyloglucan Phanerodontia chrysosporium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel45A
-
 Phanerodontia chrysosporium
3.2.1.4 EgV
-
 Phanerodontia chrysosporium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 70
-
-
 Phanerodontia chrysosporium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4
-
-
 Phanerodontia chrysosporium