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Literature summary extracted from

  • Jiang, M.; Lue, S.; Zhang, Y.
    Characterization of juvenile hormone related genes regulating cantharidin biosynthesis in Epicauta chinensis (2017), Sci. Rep., 7, 2308 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.127 gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Epicauta chinensis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.127 additional information EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection Epicauta chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.127 membrane the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158 Epicauta chinensis 16020
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.127 methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2 Epicauta chinensis
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 juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.127 Epicauta chinensis A0A2C9DK12
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.127 head expression level of EcMFE is significantly high in the head of males Epicauta chinensis
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1.14.14.127 additional information temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating Epicauta chinensis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.127 methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
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 Epicauta chinensis juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.127 ? x * 54810, sequence calculation Epicauta chinensis

Synonyms

EC Number Synonyms Comment Organism
1.14.14.127 EcMFE
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 Epicauta chinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.127 cytochrome P450 cytochrome P450 conserved site is located at amino acids 432-441 Epicauta chinensis
1.14.14.127 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Epicauta chinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.14.127 Epicauta chinensis sequence calculation
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 8.29

General Information

EC Number General Information Comment Organism
1.14.14.127 malfunction interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating Epicauta chinensis
1.14.14.127 metabolism juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III Epicauta chinensis