BRENDA - Enzyme Database

Characterization of juvenile hormone related genes regulating cantharidin biosynthesis in Epicauta chinensis

Jiang, M.; Lue, S.; Zhang, Y.; Sci. Rep. 7, 2308 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.13.202
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
1.14.14.127
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.13.202
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
1.14.14.127
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.13.202
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
1.14.14.127
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
Epicauta chinensis
-
juvenile hormone III + NADP+ + H2O
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Epicauta chinensis
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.13.202
Epicauta chinensis
A0A2C9DK12
-
-
1.14.14.127
Epicauta chinensis
A0A2C9DK12
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.202
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
1.14.13.202
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
1.14.14.127
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
1.14.14.127
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
-
746473
Epicauta chinensis
juvenile hormone III + NADP+ + H2O
-
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
746473
Epicauta chinensis
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.13.202
?
x * 54810, sequence calculation
Epicauta chinensis
1.14.14.127
?
x * 54810, sequence calculation
Epicauta chinensis
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.13.202
EcMFE
-
Epicauta chinensis
1.14.14.127
EcMFE
-
Epicauta chinensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.202
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
1.14.13.202
NADPH
-
Epicauta chinensis
1.14.14.127
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
1.14.14.127
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Epicauta chinensis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.13.202
Epicauta chinensis
sequence calculation
-
8.29
1.14.14.127
Epicauta chinensis
sequence calculation
-
8.29
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.202
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
1.14.14.127
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.202
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
1.14.13.202
NADPH
-
Epicauta chinensis
1.14.14.127
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
1.14.14.127
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Epicauta chinensis
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.13.202
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
1.14.14.127
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.13.202
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
1.14.14.127
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
Epicauta chinensis
-
juvenile hormone III + NADP+ + H2O
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Epicauta chinensis
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.202
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
1.14.13.202
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
1.14.14.127
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
1.14.14.127
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
-
746473
Epicauta chinensis
juvenile hormone III + NADP+ + H2O
-
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
746473
Epicauta chinensis
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.13.202
?
x * 54810, sequence calculation
Epicauta chinensis
1.14.14.127
?
x * 54810, sequence calculation
Epicauta chinensis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.13.202
Epicauta chinensis
sequence calculation
-
8.29
1.14.14.127
Epicauta chinensis
sequence calculation
-
8.29
General Information
EC Number
General Information
Commentary
Organism
1.14.13.202
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
1.14.13.202
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis
1.14.14.127
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
1.14.14.127
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.13.202
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
1.14.13.202
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis
1.14.14.127
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
1.14.14.127
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis