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Literature summary extracted from

  • Christensen, J.B.; Soares da Costa, T.P.; Faou, P.; Pearce, F.G.; Panjikar, S.; Perugini, M.A.
    Structure and function of cyanobacterial DHDPS and DHDPR (2016), Sci. Rep., 6, 37111 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.8 expression in Escherichia coli as His-tagged construct Trichormus variabilis
4.3.3.7 expression in Escherichia coli as His-tagged construct Trichormus variabilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.8 crystal structure is determined at 1.92 A Trichormus variabilis
4.3.3.7 crystal structure is determined at 1.92 A Trichormus variabilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate when NADPH is employed as the cofactor Trichormus variabilis
4.3.3.7 (S)-lysine
-
 Trichormus variabilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.8 0.31
-
 NADH 30°C, pH not specified in the publication Trichormus variabilis
1.17.1.8 3.6
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate 30°C, pH not specified in the publication Trichormus variabilis
4.3.3.7 0.14
-
 L-aspartate-4-semialdehyde 30°C, pH not specified in the publication Trichormus variabilis
4.3.3.7 0.41
-
 pyruvate 30°C, pH not specified in the publication Trichormus variabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.8 139000
-
 analytical ultracentrifugation Trichormus variabilis
4.3.3.7 147000
-
 analytical ultracentrifugation Trichormus variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.8 Trichormus variabilis Q3M723
-
-
4.3.3.7 Trichormus variabilis Q3MFY8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.7
-
 Trichormus variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
 Trichormus variabilis (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
 ?
4.3.3.7 pyruvate + L-aspartate-4-semialdehyde
-
 Trichormus variabilis (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.8 homotetramer
-
 Trichormus variabilis
4.3.3.7 homotetramer
-
 Trichormus variabilis

Synonyms

EC Number Synonyms Comment Organism
1.17.1.8 DHDPR
-
 Trichormus variabilis
4.3.3.7 DHDPS
-
 Trichormus variabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.8 30
-
 assay at Trichormus variabilis
4.3.3.7 30
-
 assay at Trichormus variabilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.3.3.7 0.068
-
 30°C, pH not specified in the publication Trichormus variabilis (S)-lysine