BRENDA - Enzyme Database show

Structure and function of cyanobacterial DHDPS and DHDPR

Christensen, J.B.; Soares da Costa, T.P.; Faou, P.; Pearce, F.G.; Panjikar, S.; Perugini, M.A.; Sci. Rep. 6, 37111 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.17.1.8
expression in Escherichia coli as His-tagged construct
Trichormus variabilis
4.3.3.7
expression in Escherichia coli as His-tagged construct
Trichormus variabilis
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.17.1.8
crystal structure is determined at 1.92 A
Trichormus variabilis
4.3.3.7
crystal structure is determined at 1.92 A
Trichormus variabilis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.8
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
when NADPH is employed as the cofactor
Trichormus variabilis
4.3.3.7
(S)-lysine
-
Trichormus variabilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.8
0.31
-
NADH
30°C, pH not specified in the publication
Trichormus variabilis
1.17.1.8
3.6
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
30°C, pH not specified in the publication
Trichormus variabilis
4.3.3.7
0.14
-
L-aspartate-4-semialdehyde
30°C, pH not specified in the publication
Trichormus variabilis
4.3.3.7
0.41
-
pyruvate
30°C, pH not specified in the publication
Trichormus variabilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.8
139000
-
analytical ultracentrifugation
Trichormus variabilis
4.3.3.7
147000
-
analytical ultracentrifugation
Trichormus variabilis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.1.8
Trichormus variabilis
Q3M723
-
-
4.3.3.7
Trichormus variabilis
Q3MFY8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Trichormus variabilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.8
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
746463
Trichormus variabilis
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746463
Trichormus variabilis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.1.8
homotetramer
-
Trichormus variabilis
4.3.3.7
homotetramer
-
Trichormus variabilis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.8
30
-
assay at
Trichormus variabilis
4.3.3.7
30
-
assay at
Trichormus variabilis
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.3.3.7
0.068
-
30°C, pH not specified in the publication
Trichormus variabilis
(S)-lysine
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.1.8
expression in Escherichia coli as His-tagged construct
Trichormus variabilis
4.3.3.7
expression in Escherichia coli as His-tagged construct
Trichormus variabilis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.17.1.8
crystal structure is determined at 1.92 A
Trichormus variabilis
4.3.3.7
crystal structure is determined at 1.92 A
Trichormus variabilis
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.3.3.7
0.068
-
30°C, pH not specified in the publication
Trichormus variabilis
(S)-lysine
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.17.1.8
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
when NADPH is employed as the cofactor
Trichormus variabilis
4.3.3.7
(S)-lysine
-
Trichormus variabilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.17.1.8
0.31
-
NADH
30°C, pH not specified in the publication
Trichormus variabilis
1.17.1.8
3.6
-
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
30°C, pH not specified in the publication
Trichormus variabilis
4.3.3.7
0.14
-
L-aspartate-4-semialdehyde
30°C, pH not specified in the publication
Trichormus variabilis
4.3.3.7
0.41
-
pyruvate
30°C, pH not specified in the publication
Trichormus variabilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.17.1.8
139000
-
analytical ultracentrifugation
Trichormus variabilis
4.3.3.7
147000
-
analytical ultracentrifugation
Trichormus variabilis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Trichormus variabilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.1.8
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
746463
Trichormus variabilis
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746463
Trichormus variabilis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.1.8
homotetramer
-
Trichormus variabilis
4.3.3.7
homotetramer
-
Trichormus variabilis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.17.1.8
30
-
assay at
Trichormus variabilis
4.3.3.7
30
-
assay at
Trichormus variabilis