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Literature summary extracted from

  • Roovers, M.; Oudjama, Y.; Fislage, M.; Bujnicki, J.; Vers้es, W.; Droogmans, L.
    The open reading frame TTC1157 of Thermus thermophilus HB27 encodes the methyltransferase forming N 2-methylguanosine at position 6 in tRNA (2012), RNA, 18, 815-824 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.256 expression in Escherichia coli Rosetta (DE3) Thermus thermophilus
2.1.1.256 expression in Escherichia coli Rosetta (DE3) Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.256 Pyrococcus furiosus Q8U248
-
-
2.1.1.256 Thermus thermophilus Q72IH5
-
-
2.1.1.256 Thermus thermophilus DSM 7039 Q72IH5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.256
-
 Thermus thermophilus
2.1.1.256
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Thermus thermophilus S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Pyrococcus furiosus S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNA(Phe) production of a small amount of N2-dimethylguanine6 in tRNA(Phe) Thermus thermophilus DSM 7039 S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNA(Phe)
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.256 monomer 1 * 39143, calculated from sequence Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.256 PF1002
-
 Pyrococcus furiosus
2.1.1.256 TrmN
-
 Thermus thermophilus
2.1.1.256 tRNA (m2G6) methyltransferase
-
 Thermus thermophilus
2.1.1.256 tRNA (m2G6) methyltransferase
-
 Pyrococcus furiosus
2.1.1.256 TTC1157
-
 Thermus thermophilus

General Information

EC Number General Information Comment Organism
2.1.1.256 malfunction inactivation of the trmN gene leads to a total absence of N2-methylguanine in tRNA but did not affect cell growth or the formation of other modified nucleosides in tRNA(Phe). Therefore, m2G6 does not appear to be involved in an essential function Thermus thermophilus