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Literature summary extracted from

  • Vijayakumar, R.; Tripathi, T.
    Soluble expression and purification of a full-length asparaginyl tRNA synthetase from Fasciola gigantica (2018), Protein Expr. Purif., 143, 9-13 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.22 expressed in Escherichia coli M15 cells Fasciola gigantica
6.3.5.6 expression in Escherichia coli Fasciola gigantica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.22 132000
-
 gel filtration Fasciola gigantica
6.3.5.6 132000
-
 gel filtration Fasciola gigantica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn Fasciola gigantica
-
 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.22 Fasciola gigantica
-
-
-
6.3.5.6 Fasciola gigantica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.22 Ni-NTA column chromatography Fasciola gigantica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.22 ATP + L-asparagine + tRNAAsn
-
 Fasciola gigantica AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.22 homodimer 2 * 61000, His6-tagged enzyme, SDS-PAGE Fasciola gigantica
6.3.5.6 dimer 2 * 61000, recombinant protein including His-tag, calculated and SDS-PAGE Fasciola gigantica

Synonyms

EC Number Synonyms Comment Organism
6.1.1.22 asparaginyl tRNA synthetase
-
 Fasciola gigantica
6.1.1.22 NRS
-
 Fasciola gigantica
6.3.5.6 NRS
-
 Fasciola gigantica

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.22 ATP
-
 Fasciola gigantica