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Literature summary extracted from

  • Kumar, A.; Sharma, M.; Bhardwaj, P.; Vats, S.; Singh, D.; Kumar, S.
    Copper, zinc superoxide dismutase from Caragana jubata a thermostable enzyme that functions under a broad pH and temperature window (2016), Process Biochem., 51, 1434-1444 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain M15 Caragana jubata

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 H2O2
-
 Caragana jubata
1.15.1.1 KCN
-
 Caragana jubata
1.15.1.1 additional information the enzyme is resistant to denaturation by sodium dodecyl sulfate (SDS) and urea Caragana jubata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 soluble Cj-Cu,Zn SOD is a hydrophilic protein with no transmembrane domains Caragana jubata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu,Zn SOD Caragana jubata
1.15.1.1 Zn2+ a Cu,Zn SOD Caragana jubata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Caragana jubata
-
 O2 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Caragana jubata A5HIG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography, dialysis, and ultrafiltration Caragana jubata

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 1547
-
 purified recombinant enzyme, 0°C, pH 7.8 Caragana jubata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
 Caragana jubata O2 + H2O2
-
 ?
1.15.1.1 additional information enzyme activity assay method using riboflavin and nitroblue tetrazolium (NBT) reduction Caragana jubata ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 17500, His-tagged enzyme, SDS-PAGE, 2 * 15160, sequence calculation Caragana jubata
1.15.1.1 monomer 1 * 17500, His-tagged enzyme, SDS-PAGE, 1 * 15160, sequence calculation Caragana jubata
1.15.1.1 More the enzyme exists in both monomeric and dimeric forms, the latter being more active. Circular dichroic spectroscopy analysis confirms the thermostable nature of Cj-Cu,Zn SOD, secondary structure analysis, overview Caragana jubata

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Cj-Cu, Zn SOD
-
 Caragana jubata
1.15.1.1 Cu, Zn superoxide dismutase
-
 Caragana jubata
1.15.1.1 SOD
-
 Caragana jubata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1
-
 4
-
 Caragana jubata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1
-
 80 activity range between -10-80°C Caragana jubata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 additional information
-
 circular dichroic spectroscopy analysis confirms the thermostable nature of Cj-Cu,Zn SOD. Thermal inactivation first-order kinetics Caragana jubata
1.15.1.1 80
-
 recombinant enzyme SOD Cj-Cu,Zn SOD shows a residual SOD activity of 26.07% after being heated for 160 min Caragana jubata
1.15.1.1 121
-
 Cj-Cu,Zn SOD retains 50% of the maximum activity after autoclaving, autoclaved Ca-Cu,Zn SOD exhibits 40% of maximum activity of unautoclaved enzyme at 20°C and 30°C. Thermal inactivation kinetics of Ca-Cu,Zn SOD, overview Caragana jubata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.5
-
-
 Caragana jubata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 6 9 activity range Caragana jubata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 3 10 purified recombinant His-tagged enzyme, stable for 2 and 24 h of incubation,after which it retains over 80% activity, 20–30% decrease in activity is observed at pH 10.0 Caragana jubata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.15.1.1 Caragana jubata sequence calculation
-
 5.82