EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.14.86 | synthesis | when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate | Arabidopsis thaliana |
1.14.14.86 | synthesis | when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate | Oryza sativa Japonica Group |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.14.86 | expression in Escherichia coli | Arabidopsis thaliana |
1.14.14.86 | expression in Escherichia coli | Oryza sativa Japonica Group |
1.14.14.86 | gene CYP701A3, phylogenetic analysis | Arabidopsis thaliana |
1.14.14.86 | gene CYP701A6, phylogenetic analysis | Oryza sativa Japonica Group |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.14.86 | 0.03 | - |
isopimara-7,15-diene | pH and temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.03 | - |
isopimara-8,15-diene | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.046 | - |
ent-sandaracopimaradiene | pH and temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.046 | - |
ent-sandaracopimaradiene | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.05 | - |
ent-cassadiene | pH and temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.05 | - |
syn-aphidicolene | pH and temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.05 | - |
ent-cassadiene | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
1.14.14.86 | 0.05 | - |
syn-aphidicolene | pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.14.86 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.86 | ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa Japonica Group | - |
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa Japonica Group | - |
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Arabidopsis thaliana | overall reaction | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Oryza sativa Japonica Group | overall reaction | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | Arabidopsis thaliana | - |
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa Japonica Group | - |
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.14.86 | Arabidopsis thaliana | Q93ZB2 | - |
- |
1.14.14.86 | Oryza sativa Japonica Group | Q5Z5R4 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.14.86 | ent-beyerene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa Japonica Group | ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Oryza sativa Japonica Group | ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa Japonica Group | ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa Japonica Group | ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Oryza sativa Japonica Group | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Arabidopsis thaliana | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | overall reaction | Oryza sativa Japonica Group | ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Oryza sativa Japonica Group | ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
1.14.14.86 | ent-sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | ent-sandaracopimaradien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Oryza sativa Japonica Group | ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2 | low activity | Oryza sativa Japonica Group | ? | - |
? | |
1.14.14.86 | isopimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | isopimara-8,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | 3beta-hydroxyisopimara-8,15-diene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | isopimara-8,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | additional information | use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, and ent-kauran-16beta-ol | Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | additional information | use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, ent-kauran-16beta-ol, ent-cassadiene, ent-sandaracopimaradiene, syn-aphidicolene, isopimara-7,15-diene, ent-beyerene, syn-pimara-7,15-diene, syn-stemarene, syn-stemodene, syn-labdatriene, syn-pimara-9(11),15-diene, isopimara-8,15-diene, sandaracopimaradiene | Oryza sativa Japonica Group | ? | - |
? | |
1.14.14.86 | sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | 3beta-hydroxysandaracopimaradiene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-aphidicolene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | 3beta-hydroxy-syn-aphidicolene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | syn-aphidicolene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-labdatriene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-pimara-7,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Arabidopsis thaliana | 3beta-hydroxy-syn-pimaradiene + 2alpha-hydroxy-syn-pimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? | |
1.14.14.86 | syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-pimara-9(11),15-diene + [reduced NADPH-hemoprotein reductase] + O2 | low activity | Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-stemarene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? | |
1.14.14.86 | syn-stemodene + [reduced NADPH-hemoprotein reductase] + O2 | - |
Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.14.86 | AtKO | - |
Arabidopsis thaliana |
1.14.14.86 | CYP701A6 | - |
Oryza sativa Japonica Group |
1.14.14.86 | ent-kaurene oxidase 2 | - |
Oryza sativa Japonica Group |
1.14.14.86 | KO2 | - |
Arabidopsis thaliana |
1.14.14.86 | KO2 | - |
Oryza sativa Japonica Group |
1.14.14.86 | OsKO2 | - |
Oryza sativa Japonica Group |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.14.86 | cytochrome P450 | - |
Arabidopsis thaliana | |
1.14.14.86 | cytochrome P450 | - |
Oryza sativa Japonica Group | |
1.14.14.86 | NADPH | - |
Arabidopsis thaliana | |
1.14.14.86 | NADPH | - |
Oryza sativa Japonica Group |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.14.86 | additional information | a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview | Arabidopsis thaliana |
1.14.14.86 | additional information | a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview | Oryza sativa Japonica Group |
1.14.14.86 | physiological function | the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin | Arabidopsis thaliana |
1.14.14.86 | physiological function | the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin | Oryza sativa Japonica Group |