BRENDA - Enzyme Database

Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

Mafu, S.; Jia, M.; Zi, J.; Morrone, D.; Wu, Y.; Xu, M.; Hillwig, M.L.; Peters, R.J.; Proc. Natl. Acad. Sci. USA 113, 2526-2531 (2016)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.14.14.86
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
Arabidopsis thaliana
1.14.14.86
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
Oryza sativa Japonica Group
Cloned(Commentary)
EC Number
Commentary
Organism
1.14.14.86
expression in Escherichia coli; gene CYP701A3, phylogenetic analysis
Arabidopsis thaliana
1.14.14.86
expression in Escherichia coli; gene CYP701A6, phylogenetic analysis
Oryza sativa Japonica Group
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.86
0.03
-
isopimara-7,15-diene
pH and temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.03
-
isopimara-8,15-diene
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.046
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.05
-
ent-cassadiene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.05
-
syn-aphidicolene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.86
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.86
Arabidopsis thaliana
Q93ZB2
-
-
1.14.14.86
Oryza sativa Japonica Group
Q5Z5R4
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.86
ent-beyerene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-sandaracopimaradien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
low activity
746311
Oryza sativa Japonica Group
?
-
-
-
?
1.14.14.86
isopimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
isopimara-8,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxyisopimara-8,15-diene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
isopimara-8,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
additional information
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, and ent-kauran-16beta-ol
746311
Arabidopsis thaliana
?
-
-
-
-
1.14.14.86
additional information
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, ent-kauran-16beta-ol, ent-cassadiene, ent-sandaracopimaradiene, syn-aphidicolene, isopimara-7,15-diene, ent-beyerene, syn-pimara-7,15-diene, syn-stemarene, syn-stemodene, syn-labdatriene, syn-pimara-9(11),15-diene, isopimara-8,15-diene, sandaracopimaradiene
746311
Oryza sativa Japonica Group
?
-
-
-
-
1.14.14.86
sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxysandaracopimaradiene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-aphidicolene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxy-syn-aphidicolene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
syn-aphidicolene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-labdatriene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-pimara-7,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxy-syn-pimaradiene + 2alpha-hydroxy-syn-pimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-pimara-9(11),15-diene + [reduced NADPH-hemoprotein reductase] + O2
low activity
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-stemarene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-stemodene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.86
cytochrome P450
-
Arabidopsis thaliana
1.14.14.86
cytochrome P450
-
Oryza sativa Japonica Group
1.14.14.86
NADPH
-
Arabidopsis thaliana
1.14.14.86
NADPH
-
Oryza sativa Japonica Group
Application (protein specific)
EC Number
Application
Commentary
Organism
1.14.14.86
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
Arabidopsis thaliana
1.14.14.86
synthesis
when KO2 is coexpressed in Escherichia coli engineered to produce ent-kaurene, turnover to entkauren-19-oic acid is observed. In addition, KO2 reacts with ent-isokaurene, and the closely related ent-trachylobane, converting the C4alpha methyl to a mixture of the alcohol and further oxidized carboxylic acid with each substrate
Oryza sativa Japonica Group
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.14.86
expression in Escherichia coli; gene CYP701A3, phylogenetic analysis
Arabidopsis thaliana
1.14.14.86
expression in Escherichia coli; gene CYP701A6, phylogenetic analysis
Oryza sativa Japonica Group
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.86
cytochrome P450
-
Arabidopsis thaliana
1.14.14.86
cytochrome P450
-
Oryza sativa Japonica Group
1.14.14.86
NADPH
-
Arabidopsis thaliana
1.14.14.86
NADPH
-
Oryza sativa Japonica Group
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.86
0.03
-
isopimara-7,15-diene
pH and temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.03
-
isopimara-8,15-diene
pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.046
-
ent-sandaracopimaradiene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.05
-
ent-cassadiene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
1.14.14.86
0.05
-
syn-aphidicolene
pH and temperature not specified in the publication; pH not specified in the publication, temperature not specified in the publication
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.86
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
overall reaction
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.86
ent-beyerene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-cassadiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-cassadien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-cassadiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-isokaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-isokaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-isokaurene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-isokauren-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-al + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-en-19-ol + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-al + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
746311
Arabidopsis thaliana
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-kaur-16-ene + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
746311
Oryza sativa Japonica Group
ent-kaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-sandaracopimaradien-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-trachylobane + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Oryza sativa Japonica Group
ent-trachyloban-19-oic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
ent-trachylobane + [reduced NADPH-hemoprotein reductase] + O2
low activity
746311
Oryza sativa Japonica Group
?
-
-
-
?
1.14.14.86
isopimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
isopimara-8,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxyisopimara-8,15-diene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
isopimara-8,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
additional information
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, and ent-kauran-16beta-ol
746311
Arabidopsis thaliana
?
-
-
-
-
1.14.14.86
additional information
use of combinatorial biosynthesis enabled analysis of more than 20 potential substrates, starting with (E,E,E)-geranylgeranyl diphosphate, as well as structural characterization of 12 resulting unknown products, determination of hydroxylation positions, structure-function relationship for the activity, and substrate specificity, overview. No activity with ent-pimara-8(14),15-diene, pimara-8,15-diene, miltiradiene, abietaenol, sclarene, labdadienol, ent-kauran-16beta-ol, ent-cassadiene, ent-sandaracopimaradiene, syn-aphidicolene, isopimara-7,15-diene, ent-beyerene, syn-pimara-7,15-diene, syn-stemarene, syn-stemodene, syn-labdatriene, syn-pimara-9(11),15-diene, isopimara-8,15-diene, sandaracopimaradiene
746311
Oryza sativa Japonica Group
?
-
-
-
-
1.14.14.86
sandaracopimaradiene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxysandaracopimaradiene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
sandaracopimaradiene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-aphidicolene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxy-syn-aphidicolene + 2alpha-hydroxysandaracopimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
syn-aphidicolene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-labdatriene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-pimara-7,15-diene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746311
Arabidopsis thaliana
3beta-hydroxy-syn-pimaradiene + 2alpha-hydroxy-syn-pimaradiene + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
1.14.14.86
syn-pimara-7,15-diene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-pimara-9(11),15-diene + [reduced NADPH-hemoprotein reductase] + O2
low activity
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-stemarene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
1.14.14.86
syn-stemodene + [reduced NADPH-hemoprotein reductase] + O2
-
746311
Arabidopsis thaliana
?
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.14.86
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
Arabidopsis thaliana
1.14.14.86
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
Oryza sativa Japonica Group
1.14.14.86
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
Arabidopsis thaliana
1.14.14.86
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
Oryza sativa Japonica Group
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.86
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
Arabidopsis thaliana
1.14.14.86
additional information
a modular metabolic engineering system is used in a combinatorial biosynthetic approach to detect substrates of ent-kaurene oxidase, overview
Oryza sativa Japonica Group
1.14.14.86
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
Arabidopsis thaliana
1.14.14.86
physiological function
the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), is involved in production of the diterpenoid plant hormone gibberellin
Oryza sativa Japonica Group