Any feedback?
Literature summary extracted from
- Heterologous production of extreme alkaline thermostable NAD+-dependent formate dehydrogenase with wide-range pH activity from Myceliophthora thermophila (2017), Proc. Biochem., 61, 110-118 .
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.17.1.9 | biofuel production | potential applications in NAD(H)-dependent industrial biocatalysis as well as in the production of renewable fuels and chemicals from carbon dioxide. Formate dehydrogenase from Myceliophthora thermophile possess a huge potential for CO2 reduction or NADH generation and under extreme alkaline conditions | Thermothelomyces thermophilus |
| 1.17.1.9 | synthesis | potential applications in NAD(H)-dependent industrial biocatalysis as well as in the production of renewable fuels and chemicals from carbon dioxide. Formate dehydrogenase from Myceliophthora thermophile possess a huge potential for CO2 reduction or NADH generation and under extreme alkaline conditions | Thermothelomyces thermophilus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | expressed in Escherichia coli, as 6His-tagged protein | Thermothelomyces thermophilus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.18 | - |
NADH | 25°C, pH not specified in the publication | Thermothelomyces thermophilus |  |
| 1.17.1.9 | 0.43 | - |
CO2 | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 1.2 | - |
NAD+ | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 7.2 | - |
formate | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.17.1.9 | Thermothelomyces thermophilus | G2QB71 | - |
- |
| 1.17.1.9 | Thermothelomyces thermophilus ATCC 42464 | G2QB71 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.17.1.9 | - |
Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.17.1.9 | formate + NAD+ | - |
Thermothelomyces thermophilus | CO2 + NADH + H+ | - |
r | |
| 1.17.1.9 | formate + NAD+ | - |
Thermothelomyces thermophilus ATCC 42464 | CO2 + NADH + H+ | - |
r | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 35 | - |
- |
Thermothelomyces thermophilus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 25 | 49 | 25°C: about 75% of maximal activity, 49°C: about 40% of maximal activity | Thermothelomyces thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 48 | - |
Tm-value | Thermothelomyces thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.03 | - |
NADH | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.1 | - |
CO2 | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.32 | - |
formate | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.76 | - |
NAD+ | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.17.1.9 | 5.5 | - |
conversion of carbon dioxide to formate using as cofactor NADH | Thermothelomyces thermophilus |
| 1.17.1.9 | 10.5 | - |
conversion of formate to carbon dioxide | Thermothelomyces thermophilus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.17.1.9 | NAD+ | - |
Thermothelomyces thermophilus | |
| 1.17.1.9 | NADH | - |
Thermothelomyces thermophilus | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.17.1.9 | Thermothelomyces thermophilus | calculated from sequence | - |
6.15 |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.17.1.9 | 0.04 | - |
formate | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.17 | - |
NADH | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.23 | - |
CO2 | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
| 1.17.1.9 | 0.63 | - |
NAD+ | 25°C, pH not specified in the publication | Thermothelomyces thermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
