BRENDA - Enzyme Database

Polyphenoloxidase activity and browning in fresh-cut Rocha pear as affected by pH, phenolic substrates, and antibrowning additives

Gomes, M.; Vieira, T.; Fundo, J.; Almeida, D.; Postharvest Biol. Technol. 91, 32-38 (2014)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
4-hexylresorcinol
-
Pyrus communis subsp. caucasica
1.14.18.1
ascorbic acid
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium ascorbate
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium chloride
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium lactate
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium propionate
-
Pyrus communis subsp. caucasica
1.14.18.1
additional information
pH-dependent inhibition of the enzyme
Pyrus communis subsp. caucasica
1.14.18.1
N-acetyl-L-cysteine
-
Pyrus communis subsp. caucasica
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.18.1
(+)-catechin hydrate + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
1.14.18.1
2 4-methylcatechol + O2
Pyrus communis subsp. caucasica
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
?
1.14.18.1
2 catechol + O2
Pyrus communis subsp. caucasica
-
2 1,2-benzoquinone + 2 H2O
-
-
?
1.14.18.1
2 L-dopa + O2
Pyrus communis subsp. caucasica
-
2 dopaquinone + 2 H2O
-
-
?
1.14.18.1
caffeic acid + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
1.14.18.1
chlorogenic acid + O2
Pyrus communis subsp. caucasica
best substrate
? + 2 H2O
-
-
?
1.14.18.1
L-tyrosine + O2
Pyrus communis subsp. caucasica
-
dopaquinone + H2O
-
-
?
1.14.18.1
pyrogallol + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.18.1
Pyrus communis subsp. caucasica
A7J0M6
cv. Rocha, from orchards located in the Western Region of Portugal
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(+)-catechin + O2
high activity
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
(+)-catechin hydrate + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
2 4-methylcatechol + O2
-
746276
Pyrus communis subsp. caucasica
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 4-methylcatechol + O2
high activity
746276
Pyrus communis subsp. caucasica
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 catechol + O2
-
746276
Pyrus communis subsp. caucasica
2 1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 catechol + O2
high activity
746276
Pyrus communis subsp. caucasica
2 1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 L-dopa + O2
-
746276
Pyrus communis subsp. caucasica
2 dopaquinone + 2 H2O
-
-
-
?
1.14.18.1
caffeic acid + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
chlorogenic acid + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
chlorogenic acid + O2
best substrate
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
L-tyrosine + O2
-
746276
Pyrus communis subsp. caucasica
dopaquinone + H2O
-
-
-
?
1.14.18.1
L-tyrosine + O2
low activity
746276
Pyrus communis subsp. caucasica
dopaquinone + H2O
-
-
-
?
1.14.18.1
additional information
substrate specificity, overview
746276
Pyrus communis subsp. caucasica
?
-
-
-
-
1.14.18.1
pyrogallol + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
pyrogallol + O2
low activity
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
25
-
assay at
Pyrus communis subsp. caucasica
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
additional information
-
pH optima for PPO activity depend on the phenolic substrates, overview
Pyrus communis subsp. caucasica
1.14.18.1
5
-
with catechol
Pyrus communis subsp. caucasica
1.14.18.1
6
-
with 4-methylcatechol
Pyrus communis subsp. caucasica
1.14.18.1
7
-
with dopamine, caffeic acid, and catechin
Pyrus communis subsp. caucasica
1.14.18.1
8
-
with pyrogallol
Pyrus communis subsp. caucasica
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
3
8
PPO activity increases with pH from pH 3.0 to pH 7.0 and decreases slightly as pH increases from pH 7.0 to pH 8.0, but this general pattern is affected by the substrate, pH optima for PPO activity depend on the phenolic substrates, detailed overview
Pyrus communis subsp. caucasica
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.18.1
4-hexylresorcinol
-
Pyrus communis subsp. caucasica
1.14.18.1
ascorbic acid
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium ascorbate
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium chloride
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium lactate
-
Pyrus communis subsp. caucasica
1.14.18.1
calcium propionate
-
Pyrus communis subsp. caucasica
1.14.18.1
additional information
pH-dependent inhibition of the enzyme
Pyrus communis subsp. caucasica
1.14.18.1
N-acetyl-L-cysteine
-
Pyrus communis subsp. caucasica
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.18.1
(+)-catechin hydrate + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
1.14.18.1
2 4-methylcatechol + O2
Pyrus communis subsp. caucasica
-
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
?
1.14.18.1
2 catechol + O2
Pyrus communis subsp. caucasica
-
2 1,2-benzoquinone + 2 H2O
-
-
?
1.14.18.1
2 L-dopa + O2
Pyrus communis subsp. caucasica
-
2 dopaquinone + 2 H2O
-
-
?
1.14.18.1
caffeic acid + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
1.14.18.1
chlorogenic acid + O2
Pyrus communis subsp. caucasica
best substrate
? + 2 H2O
-
-
?
1.14.18.1
L-tyrosine + O2
Pyrus communis subsp. caucasica
-
dopaquinone + H2O
-
-
?
1.14.18.1
pyrogallol + O2
Pyrus communis subsp. caucasica
-
? + 2 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.18.1
(+)-catechin + O2
high activity
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
(+)-catechin hydrate + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
2 4-methylcatechol + O2
-
746276
Pyrus communis subsp. caucasica
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 4-methylcatechol + O2
high activity
746276
Pyrus communis subsp. caucasica
2 4-methyl-1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 catechol + O2
-
746276
Pyrus communis subsp. caucasica
2 1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 catechol + O2
high activity
746276
Pyrus communis subsp. caucasica
2 1,2-benzoquinone + 2 H2O
-
-
-
?
1.14.18.1
2 L-dopa + O2
-
746276
Pyrus communis subsp. caucasica
2 dopaquinone + 2 H2O
-
-
-
?
1.14.18.1
caffeic acid + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
chlorogenic acid + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
chlorogenic acid + O2
best substrate
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
L-tyrosine + O2
-
746276
Pyrus communis subsp. caucasica
dopaquinone + H2O
-
-
-
?
1.14.18.1
L-tyrosine + O2
low activity
746276
Pyrus communis subsp. caucasica
dopaquinone + H2O
-
-
-
?
1.14.18.1
additional information
substrate specificity, overview
746276
Pyrus communis subsp. caucasica
?
-
-
-
-
1.14.18.1
pyrogallol + O2
-
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
1.14.18.1
pyrogallol + O2
low activity
746276
Pyrus communis subsp. caucasica
? + 2 H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.18.1
25
-
assay at
Pyrus communis subsp. caucasica
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.18.1
additional information
-
pH optima for PPO activity depend on the phenolic substrates, overview
Pyrus communis subsp. caucasica
1.14.18.1
5
-
with catechol
Pyrus communis subsp. caucasica
1.14.18.1
6
-
with 4-methylcatechol
Pyrus communis subsp. caucasica
1.14.18.1
7
-
with dopamine, caffeic acid, and catechin
Pyrus communis subsp. caucasica
1.14.18.1
8
-
with pyrogallol
Pyrus communis subsp. caucasica
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.14.18.1
3
8
PPO activity increases with pH from pH 3.0 to pH 7.0 and decreases slightly as pH increases from pH 7.0 to pH 8.0, but this general pattern is affected by the substrate, pH optima for PPO activity depend on the phenolic substrates, detailed overview
Pyrus communis subsp. caucasica
General Information
EC Number
General Information
Commentary
Organism
1.14.18.1
physiological function
the enzyme is involved in browning of fruit slices
Pyrus communis subsp. caucasica
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.18.1
physiological function
the enzyme is involved in browning of fruit slices
Pyrus communis subsp. caucasica