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Literature summary extracted from

  • Chen, D.; Feng, J.; Huang, L.; Zhang, Q.; Wu, J.; Zhu, X.; Duan, Y.; Xu, Z.
    Identification and characterization of a new erythromycin biosynthetic gene cluster in Actinopolyspora erythraea YIM90600, a novel erythronolide-producing halophilic actinomycete isolated from salt field (2014), PLoS ONE, 9, e108129 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.35 gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001 Saccharopolyspora erythraea
1.14.15.35 gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001) Actinopolyspora erythraea

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.35 additional information construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression Saccharopolyspora erythraea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Saccharopolyspora erythraea C-6 hydroxylation erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Actinopolyspora erythraea C-6 hydroxylation erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Actinopolyspora erythraea a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Saccharopolyspora erythraea NRRL2338 C-6 hydroxylation erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Actinopolyspora erythraea YIM90600 C-6 hydroxylation erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 Actinopolyspora erythraea YIM90600 a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 additional information Actinopolyspora erythraea the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro ?
-
 ?
1.14.15.35 additional information Actinopolyspora erythraea YIM90600 the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.35 Actinopolyspora erythraea A0A099D4T8 halophilic actinomycete isolated from Baicheng salt field
-
1.14.15.35 Actinopolyspora erythraea YIM90600 A0A099D4T8 halophilic actinomycete isolated from Baicheng salt field
-
1.14.15.35 Saccharopolyspora erythraea Q00441
-
-
1.14.15.35 Saccharopolyspora erythraea NRRL2338 Q00441
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 C-6 hydroxylation Saccharopolyspora erythraea erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 C-6 hydroxylation Actinopolyspora erythraea erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 C-6 hydroxylation Saccharopolyspora erythraea NRRL2338 erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 C-6 hydroxylation Actinopolyspora erythraea YIM90600 erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B Actinopolyspora erythraea erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2 a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B Actinopolyspora erythraea YIM90600 erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.15.35 additional information the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro Actinopolyspora erythraea ?
-
 ?
1.14.15.35 additional information the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro Actinopolyspora erythraea YIM90600 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.35 eryF
-
 Saccharopolyspora erythraea
1.14.15.35 eryF
-
 Actinopolyspora erythraea
1.14.15.35 EryFAc
-
 Actinopolyspora erythraea
1.14.15.35 EryFSa
-
 Saccharopolyspora erythraea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.35 30
-
 assay at Saccharopolyspora erythraea
1.14.15.35 30
-
 assay at Actinopolyspora erythraea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.35 7.5
-
 assay at Saccharopolyspora erythraea
1.14.15.35 7.5
-
 assay at Actinopolyspora erythraea

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.35 cytochrome P450
-
 Saccharopolyspora erythraea
1.14.15.35 cytochrome P450
-
 Actinopolyspora erythraea
1.14.15.35 Ferredoxin
-
 Saccharopolyspora erythraea
1.14.15.35 Ferredoxin
-
 Actinopolyspora erythraea
1.14.15.35 ferredoxin [iron-sulfur] cluster
-
 Saccharopolyspora erythraea
1.14.15.35 ferredoxin [iron-sulfur] cluster
-
 Actinopolyspora erythraea

General Information

EC Number General Information Comment Organism
1.14.15.35 evolution the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype Saccharopolyspora erythraea
1.14.15.35 evolution the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B Actinopolyspora erythraea
1.14.15.35 metabolism the enzyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview Saccharopolyspora erythraea
1.14.15.35 metabolism the enzyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview Actinopolyspora erythraea