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Literature summary extracted from

  • Choi, J.H.; Shin, K.C.; Oh, D.K.
    An L213A variant of beta-glycosidase from Sulfolobus solfataricus with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to compound K (2018), PLoS ONE, 13, e0191018 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 expressed in Escherichia coli Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.B26 G217A the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 G217N the mutantconverts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 H342A the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) Saccharolobus solfataricus
3.2.1.B26 H342E inactive Saccharolobus solfataricus
3.2.1.B26 K219R the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) Saccharolobus solfataricus
3.2.1.B26 L213A the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol). The variant enzyme converts ginsenosides Rc and C-Mc into C-K with molar conversions of 97%, which are 1.5 and 2fold higher, respectively, than those of the wild type enzyme Saccharolobus solfataricus
3.2.1.B26 L213D the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 L213E the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 L213G the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 L213H the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 L213Q the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) Saccharolobus solfataricus
3.2.1.B26 L213R inactive Saccharolobus solfataricus
3.2.1.B26 L213S the mutant converts ginsenoside Rc to ginsenoside Rd Saccharolobus solfataricus
3.2.1.B26 L213W the mutant with increased alpha-L-arabinofuranosidase activity converts ginsenoside Rc to ginsenoside C-K (i.e. 20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus P22498
-
-
3.2.1.B26 Saccharolobus solfataricus DSM 1617 P22498
-
-
3.2.1.B28 Pyrococcus furiosus
-
-
-
3.2.1.55 Saccharolobus solfataricus P22498
-
-
3.2.1.55 Saccharolobus solfataricus DSM 1617 P22498
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.B26 0.0126
-
wild type enzyme, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.0567
-
mutant enzyme L213A, with ginsenoside C-Mc1 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.0661
-
wild type enzyme, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.0802
-
mutant enzyme L213A, with ginsenoside Rd as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.146
-
mutant enzyme L213A, with ginsenoside C-Mc as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.211
-
wild type enzyme, with ginsenoside Rd as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.52
-
wild type enzyme, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 0.54
-
mutant enzyme L213A, with ginsenoside Rb2 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 1.18
-
mutant enzyme L213A, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 1.74
-
wild type enzyme, with ginsenoside C-Y as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 4.05
-
mutant enzyme L213A, with ginsenoside C-O as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 4.24
-
wild type enzyme, with ginsenoside C-O as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 8.56
-
wild type enzyme, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 13.65
-
mutant enzyme L213A, with ginsenoside Rb1 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 24.1
-
wild type enzyme, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus
3.2.1.B26 33.4
-
mutant enzyme L213A, 1ith ginsenoside F2 as substrate, at pH 5.5 and 95°C Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 ginsenoside C-Mc + H2O
-
Saccharolobus solfataricus ?
-
?
3.2.1.B26 ginsenoside C-Mc + H2O
-
Saccharolobus solfataricus DSM 1617 ?
-
?
3.2.1.B26 ginsenoside C-Mc1 + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose + L-arabinofuranose
-
?
3.2.1.B26 ginsenoside C-O + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose + L-arabinopyranose
-
?
3.2.1.B26 ginsenoside C-Y + H2O
-
Saccharolobus solfataricus ginsenoside C-K + L-arabinopyranose
-
?
3.2.1.B26 ginsenoside F2 + H2O highest activity Saccharolobus solfataricus ginsenoside C-K + D-glucose
-
?
3.2.1.B26 ginsenoside Rb1 + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose
-
?
3.2.1.B26 ginsenoside Rb2 + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose + L-arabinopyranose
-
?
3.2.1.B26 ginsenoside Rc + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose + L-arabinofuranose
-
?
3.2.1.B26 ginsenoside Rd + H2O
-
Saccharolobus solfataricus ginsenoside C-K + D-glucose
-
?
3.2.1.B26 additional information no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc Saccharolobus solfataricus ?
-
?
3.2.1.B26 additional information the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1 Saccharolobus solfataricus ?
-
?
3.2.1.B26 additional information no activity with alpha-L-arabinofuranoside linked to ginsenoside Rc Saccharolobus solfataricus DSM 1617 ?
-
?
3.2.1.B26 additional information the specific hydrolytic activity for the wild type enzyme follows the order ginsenoside F2 > Rb1 > C-O > C-Y > Rb2 > Rd > C-Mc> C-Mc1 Saccharolobus solfataricus DSM 1617 ?
-
?
3.2.1.B28 ginsenoside Rc + H2O
-
Pyrococcus furiosus (3beta,12beta)-dammar-24-ene-3,12,20-triol + D-glucose + L-arabinofuranose
-
?
3.2.1.B28 additional information the enzyme also contains alpha-L-arabinofuranosidase activity Pyrococcus furiosus ?
-
?
3.2.1.55 ginsenoside C-Mc + H2O
-
Saccharolobus solfataricus ginsenoside C-K + alpha-L-arabinofuranose
-
?
3.2.1.55 ginsenoside C-Mc + H2O
-
Saccharolobus solfataricus DSM 1617 ginsenoside C-K + alpha-L-arabinofuranose
-
?
3.2.1.55 ginsenoside Rc + H2O
-
Saccharolobus solfataricus ginsenoside Rd + alpha-L-arabinofuranose
-
?
3.2.1.55 ginsenoside Rc + H2O
-
Saccharolobus solfataricus DSM 1617 ginsenoside Rd + alpha-L-arabinofuranose
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 beta-glycosidase
-
Saccharolobus solfataricus
3.2.1.B28 beta-glycosidase
-
Pyrococcus furiosus
3.2.1.55 L213A variant beta-glycosidase
-
Saccharolobus solfataricus