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Literature summary extracted from

  • Ravishankar, S.; Ambady, A.; Swetha, R.G.; Anbarasu, A.; Ramaiah, S.; Sambandamurthy, V.K.
    Essentiality assessment of cysteinyl and lysyl-tRNA synthetases of Mycobacterium smegmatis (2016), PLoS ONE, 11, e0147188 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.1.1.6 drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis
6.1.1.15 drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis
6.1.1.16 drug development aminoacyl-tRNA synthetases are attractive targets for the discovery of antibacterial agents Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.6 gene lysS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 Mycolicibacterium smegmatis
6.1.1.6 gene lysS2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis Mycolicibacterium smegmatis
6.1.1.15 gene proS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 from pAZI9452 vector Mycolicibacterium smegmatis
6.1.1.16 gene cysS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 from pAZI9452 vector Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.6 additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis
6.1.1.6 additional information generation of conditional expression strains, conditional expression plasmids are electroporated intoMycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis
6.1.1.15 additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis
6.1.1.16 additional information generation of conditional expression strains, conditional expression plasmids are electroporated into Mycobacterium smegmatis strain mc2155, analysis of inducer dependency of conditional expression strains, overview Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.6 membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-
6.1.1.15 membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-
6.1.1.16 membrane transmembrane segment analysis of lysyl-tRNA synthetase Mycolicibacterium smegmatis 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.6 Mg2+ required Mycolicibacterium smegmatis
6.1.1.15 Mg2+ required Mycolicibacterium smegmatis
6.1.1.16 Mg2+ required Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys Mycolicibacterium smegmatis
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys Mycobacterium leprae
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys Mycolicibacterium smegmatis mc2155 / ATCC 700084
-
 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Mycolicibacterium smegmatis A0QYV1 gene lysX or lysS2
-
6.1.1.6 Mycolicibacterium smegmatis A0R577 gene lysS
-
6.1.1.6 Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0QYV1 gene lysX or lysS2
-
6.1.1.6 Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0R577 gene lysS
-
6.1.1.15 Mycolicibacterium smegmatis A0QVM0 gene proS
-
6.1.1.15 Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0QVM0 gene proS
-
6.1.1.16 Mycobacterium leprae
-
-
-
6.1.1.16 Mycolicibacterium smegmatis
-
-
-
6.1.1.16 Mycolicibacterium smegmatis A0R578 gene cysS
-
6.1.1.16 Mycolicibacterium smegmatis mc2155 / ATCC 700084 A0R578 gene cysS
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-lysyl-tRNALys
-
 ?
6.1.1.6 ATP + L-lysine + tRNALys
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-lysyl-tRNALys
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.15 ATP + L-proline + tRNAPro
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Mycolicibacterium smegmatis AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Mycobacterium leprae AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
6.1.1.16 ATP + L-cysteine + tRNACys
-
 Mycolicibacterium smegmatis mc2155 / ATCC 700084 AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.15 More the enzyme possess motifs for a catalytic, an anti-codon binding and a tRNA editing activity, similar to the ones found in the canonical prolyl-tRNA synthetase of Escherichia coli and Mycobacterium tuberculosis Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
6.1.1.6 LysRS
-
 Mycolicibacterium smegmatis
6.1.1.6 lysS
-
 Mycolicibacterium smegmatis
6.1.1.6 lysS2
-
 Mycolicibacterium smegmatis
6.1.1.6 LysX
-
 Mycolicibacterium smegmatis
6.1.1.6 LysX UniProt Mycolicibacterium smegmatis
6.1.1.6 Lysyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
6.1.1.6 lysylphosphatidylglycerol biosynthesis bifunctional protein UniProt Mycolicibacterium smegmatis
6.1.1.6 MSMEG_3796
-
 Mycolicibacterium smegmatis
6.1.1.6 MSMEG_6094
-
 Mycolicibacterium smegmatis
6.1.1.6 MSMEI_3707
-
 Mycolicibacterium smegmatis
6.1.1.15 MSMEG_2621
-
 Mycolicibacterium smegmatis
6.1.1.15 Prolyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
6.1.1.15 ProRS
-
 Mycolicibacterium smegmatis
6.1.1.15 proS
-
 Mycolicibacterium smegmatis
6.1.1.16 CysRS
-
 Mycolicibacterium smegmatis
6.1.1.16 CysRS
-
 Mycobacterium leprae
6.1.1.16 cysS
-
 Mycolicibacterium smegmatis
6.1.1.16 cysteinyl tRNA synthetase
-
 Mycolicibacterium smegmatis
6.1.1.16 cysteinyl tRNA synthetase
-
 Mycobacterium leprae
6.1.1.16 Cysteinyl-tRNA synthetase
-
 Mycolicibacterium smegmatis
6.1.1.16 MSMEG_6074
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.6 ATP
-
 Mycolicibacterium smegmatis
6.1.1.15 ATP
-
 Mycolicibacterium smegmatis
6.1.1.16 ATP
-
 Mycolicibacterium smegmatis
6.1.1.16 ATP
-
 Mycobacterium leprae

General Information

EC Number General Information Comment Organism
6.1.1.6 evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases. Conditional expression strains of lysyl-tRNA synthetases generated in Mycobacterium smegmatis reveals that the canonical aminoacyl-tRNA synthetase are essential, while the additional ones are not essential for the growth of Mycobacterium smegmatis. The lysyl-tRNA synthetase of Mycobacterium smegmatis belongs to the class II amino acid tRNA synthetases Mycolicibacterium smegmatis
6.1.1.6 physiological function canonical LysRS and CysRS are essential for growth of Mycobacterium smegmatis Mycolicibacterium smegmatis
6.1.1.6 physiological function Essentiality of lysyl and cysteinyl-tRNA synthetases of Mycobacterium smegmatis Mycolicibacterium smegmatis
6.1.1.15 evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases. The enzyme possess motifs for a catalytic, an anti-codon binding and a tRNA editing activity, similar to the ones found in the canonical prolyl-tRNA synthetase of Escherichia coli and Mycobacterium tuberculosis Mycolicibacterium smegmatis
6.1.1.15 additional information sequence and structural analysis of prolyl-tRNA synthetase, overview Mycolicibacterium smegmatis
6.1.1.16 evolution multiple genes encode for aminoacyl-tRNA synthetases from Mycobacterium smegmatis, e.g. glutamyl (GluRS), cysteinyl (CysRS), prolyl (ProRS) and lysyl (LysRS) tRNA synthetases Mycolicibacterium smegmatis
6.1.1.16 physiological function Essentiality of lysyl and cysteinyl-tRNA synthetases of Mycobacterium smegmatis Mycolicibacterium smegmatis
6.1.1.16 physiological function the enzyme is essential for growth Mycolicibacterium smegmatis