BRENDA - Enzyme Database show

Genome-wide association mapping identifies a new arsenate reductase enzyme critical for limiting arsenic accumulation in plants

Chao, D.Y.; Chen, Y.; Chen, J.; Shi, S.; Chen, Z.; Wang, C.; Danku, J.M.; Zhao, F.J.; Salt, D.E.; PLoS Biol. 12, e1002009 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.20.99.1
expressed in Escherichia coli W3110 cells
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Arabidopsis thaliana
-
arsenate + reduced acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.99.1
Arabidopsis thaliana
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.20.99.1
root
-
Arabidopsis thaliana
-
1.20.99.1
shoot
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.99.1
arsenite + acceptor
-
746165
Arabidopsis thaliana
arsenate + reduced acceptor
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.20.99.1
expressed in Escherichia coli W3110 cells
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.99.1
arsenite + acceptor
Arabidopsis thaliana
-
arsenate + reduced acceptor
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.20.99.1
root
-
Arabidopsis thaliana
-
1.20.99.1
shoot
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.99.1
arsenite + acceptor
-
746165
Arabidopsis thaliana
arsenate + reduced acceptor
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.20.99.1
Arabidopsis thaliana
enzyme expression is induced by arsenate
up
General Information
EC Number
General Information
Commentary
Organism
1.20.99.1
malfunction
plants lacking the enzyme lose their ability to efflux arsenite from roots, leading to both increased transport of arsenic into the central vascular tissue and on into the shoot. Loss of enzyme-encoded arsenic reduction leads to a significant increase in arsenic accumulation in shoots, causing an increased sensitivity to arsenate toxicity
Arabidopsis thaliana
1.20.99.1
physiological function
the enzyme functions to reduce arsenate to arsenite in the outer cell layer of the root, facilitating efflux of arsenic as arsenite back into the soil to limit both its accumulation in the root and transport to the shoot. Arsenate reduction by the enzyme in the pericycle may play a role in limiting arsenic loading into the xylem
Arabidopsis thaliana
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.20.99.1
malfunction
plants lacking the enzyme lose their ability to efflux arsenite from roots, leading to both increased transport of arsenic into the central vascular tissue and on into the shoot. Loss of enzyme-encoded arsenic reduction leads to a significant increase in arsenic accumulation in shoots, causing an increased sensitivity to arsenate toxicity
Arabidopsis thaliana
1.20.99.1
physiological function
the enzyme functions to reduce arsenate to arsenite in the outer cell layer of the root, facilitating efflux of arsenic as arsenite back into the soil to limit both its accumulation in the root and transport to the shoot. Arsenate reduction by the enzyme in the pericycle may play a role in limiting arsenic loading into the xylem
Arabidopsis thaliana
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.20.99.1
Arabidopsis thaliana
enzyme expression is induced by arsenate
up