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Literature summary extracted from

  • Liu, T.; Zhu, P.; Cheng, K.D.; Meng, C.; He, H.X.
    Molecular cloning, expression and characterization of hyoscyamine 6beta-hydroxylase from hairy roots of Anisodus tanguticus (2005), Planta Med., 71, 249-253 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.20.13 ascorbate in absence of ascorbate, 14% of the activity in presence of 4 mM Anisodus tanguticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.20.13 Ca2+ 5 mM, 100% inhibition Anisodus tanguticus
1.14.20.13 Cu2+ 5 mM, 100% inhibition Anisodus tanguticus
1.14.20.13 EDTA 5 mM, 61% inhibition Anisodus tanguticus
1.14.20.13 Fe3+ 5 mM, 65% inhibition Anisodus tanguticus
1.14.20.13 Mg2+ 5 mM, 14% inhibition Anisodus tanguticus
1.14.20.13 Ni2+ 5 mM, 64% inhibition Anisodus tanguticus
1.14.20.13 Zn2+ 5 mM, 66% inhibition Anisodus tanguticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.20.13 0.017
-
 (6S)-6beta-hydroxyhyoscyamine pH 7.4, 30°C Anisodus tanguticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.13 Fe2+ required, only 1.3% of maximum activity in absence of Fe2+ Anisodus tanguticus

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.13 Anisodus tanguticus Q6V0J1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.20.13 root hairy root Anisodus tanguticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.13 (6S)-6beta-hydroxyhyoscyamine + 2-oxoglutarate + O2
-
 Anisodus tanguticus scopolamine + succinate + CO2 + H2O
-
 ?
1.14.20.13 additional information enzyme catalyzes both the hydroxylation of hyoscyamine, reaction of EC 1.14.11.11, and the epoxidation of 6beta-hydroxyhyoscyamine to generate scopolamine Anisodus tanguticus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.20.13 30
-
-
 Anisodus tanguticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.20.13 7.4
-
-
 Anisodus tanguticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.20.13 5
-
 20% of maximum activity Anisodus tanguticus