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Literature summary extracted from

  • Wang, P.; Zhang, L.; Jiang, X.; Dai, X.; Xu, L.; Li, T.; Xing, D.; Li, Y.; Li, M.; Gao, L.; Xia, T.
    Evolutionary and functional characterization of leucoanthocyanidin reductases from Camellia sinensis (2018), Planta, 247, 139-154 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.3 gene LARa, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco Camellia sinensis
1.17.1.3 gene LARb, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strains EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco Camellia sinensis
1.17.1.3 gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1-mediated transformation, quantitative real-time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco Camellia sinensis
1.17.1.3 gene LARc, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli, recombinant expression in transgenic Nicotianan tabacum and Arabidopsis thaliana via Agrobacterium tumefaciens strain s EHA105 and C58C1?mediated transformation, quantitative real?time PCR enzyme expression analysis. In Arabidopsis thaliana, contents of both insoluble and soluble proanthocyanidins extracted from the seeds are reduced in the overexpressing CsLARs lines compared with wild-type, although CsLARs catalyze leucocyanidins conversion to catechin in vitro, no catechin is detected in any transgenic Arabidopsis thaliana lines. Also no proanthocyanidins are detected in the transgenic tobacco Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ Camellia sinensis
-
 (2R,3S)-catechin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.3 Camellia sinensis A0A286QXX3 cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)
-
1.17.1.3 Camellia sinensis A0A286QXY4 cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)
-
1.17.1.3 Camellia sinensis I1E425 cv. Shuchazao, TRI2043, grown in an experimental tea field at Anhui agricultural university, Hefei, China (east longitude 117.27, north latitude 31.86)
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.3 leaf
-
 Camellia sinensis
-
1.17.1.3 leaf bud
-
 Camellia sinensis
-
1.17.1.3 additional information accumulation of catechins is greater in the buds and younger leaves than in the mature leaves, stems and roots Camellia sinensis
-
1.17.1.3 root
-
 Camellia sinensis
-
1.17.1.3 stem young fine shoots Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
 Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.3 ? x * 37000, about, sequence calculation Camellia sinensis
1.17.1.3 ? x * 36000, about, sequence calculation Camellia sinensis
1.17.1.3 ? x * 43000, about, sequence calculation Camellia sinensis

Synonyms

EC Number Synonyms Comment Organism
1.17.1.3 CsLAR
-
 Camellia sinensis
1.17.1.3 LARa
-
 Camellia sinensis
1.17.1.3 LarB
-
 Camellia sinensis
1.17.1.3 LarC
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.3 NADPH
-
 Camellia sinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.17.1.3 Camellia sinensis sequence calculation
-
 5.27
1.17.1.3 Camellia sinensis sequence calculation
-
 5.34
1.17.1.3 Camellia sinensis sequence calculation
-
 5.43

General Information

EC Number General Information Comment Organism
1.17.1.3 evolution phylogenetic analysis of the LAR family, overview Camellia sinensis
1.17.1.3 evolution phylogenetic analysis of the LAR family, overview. The dicotyledonous LARs can be clustered into two subgroups, which are defined as cluster I and cluster II Camellia sinensis
1.17.1.3 malfunction overexpression of CsLAR causes a decrease in the proanthocyanidins in transgenic plants Camellia sinensis
1.17.1.3 physiological function the enzyme is required in the proanthocyanidin biosynthesis Camellia sinensis