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Literature summary extracted from

  • Zhao, N.; Zhang, Y.; Li, Q.; Li, R.; Xia, X.; Qin, X.; Guo, H.
    Identification and expression of a stearoyl-ACP desaturase gene responsible for oleic acid accumulation in Xanthoceras sorbifolia seeds (2015), Plant Physiol. Biochem., 87, 9-16 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.2 gene sad, cloning from embryo, DNA and amino acid sequence determination and analysis, quantitative and semi-quantitative real-time RT-PCR enzyme expression analysis, recombinant XsSAD expression in Escherichia coli cells resulting in increased 18:1D9 level, and confirming the biological activity of the enzyme encoded by XsSAD. Recombinant XsSAD expression in and partial complementation of Arabidopsis thaliana ssi2 mutant, via Agrobacterium strain GV3101 transformation method,control of 35S promoter partially restors the morphological phenotype and effectively increases the 18:1D9 level. The levels of other unsaturated fatty acids synthesized with 18:1D9 as the substrate also increase to some degree Xanthoceras sorbifolium

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.2 additional information XsSAD expression in the Arabidopsis thaliana ssi2 mutant partially complements the morphological phenotype of Arabidopsis thaliana ssi2 plants. Fatty acid composition of wild-type and mutant plants, overview Xanthoceras sorbifolium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.2 chloroplast stroma
-
 Xanthoceras sorbifolium 9570
-
1.14.19.2 soluble
-
 Xanthoceras sorbifolium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.2 Fe2+ ferredoxin-[iron-sulfur] cluster Xanthoceras sorbifolium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Xanthoceras sorbifolium
-
 oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.2 Xanthoceras sorbifolium A0A0K0MC84
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.2 embryo high enzyme content Xanthoceras sorbifolium
-
1.14.19.2 leaf low enzyme content Xanthoceras sorbifolium
-
1.14.19.2 additional information XsSAD Xanthoceras sorbifolia has a much higher expression in embryos than in leaves and petals. XsSAD expression also correlated well with the oleic acid, unsaturated fatty acid, and total fatty acid levels in developing embryos Xanthoceras sorbifolium
-
1.14.19.2 petal low enzyme content Xanthoceras sorbifolium
-
1.14.19.2 seed
-
 Xanthoceras sorbifolium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.2 stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Xanthoceras sorbifolium oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.2 ? x * 45510, sequence calculation Xanthoceras sorbifolium

Synonyms

EC Number Synonyms Comment Organism
1.14.19.2 SAD
-
 Xanthoceras sorbifolium
1.14.19.2 stearoyl-ACP desaturase
-
 Xanthoceras sorbifolium
1.14.19.2 XsSAD
-
 Xanthoceras sorbifolium

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.2 Ferredoxin
-
 Xanthoceras sorbifolium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.2 Xanthoceras sorbifolium sequence calculation
-
 6.24

General Information

EC Number General Information Comment Organism
1.14.19.2 malfunction fatty acid composition of wild-type and mutant plants, overview Xanthoceras sorbifolium
1.14.19.2 physiological function stearoyl-ACP desaturase is a plastid-localized soluble desaturase that catalyzes the conversion of stearic acid (18:0) to oleic acid, which plays a key role in determining the ratio of saturated to unsaturated fatty acids Xanthoceras sorbifolium