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Literature summary extracted from

  • Xiong, S.; Tian, N.; Long, J.; Chen, Y.; Qin, Y.; Feng, J.; Xiao, W.; Liu, S.
    Molecular cloning and characterization of a flavanone 3-hydroxylase gene from Artemisia annua L. (2016), Plant Physiol. Biochem., 105, 29-36 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 gene F3H, DNA and amino acid sequence determination and analysis, phylogenetic tree, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Artemisia annua

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.9 biotecnology AaF3H is a potential target for regulation of flavonoids biosynthesis in Artemisia annua through metabolic engineering Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.11.9 additional information
-
 additional information Michaelis-Menten kinetics Artemisia annua
1.14.11.9 0.218
-
 naringenin pH 8.5, 35°C, recombinant His-tagged enzyme Artemisia annua

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Fe2+ required for activity Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 naringenin + 2-oxoglutarate + O2 Artemisia annua
-
 dihydrokaempferol + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Artemisia annua
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.11.9 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Artemisia annua

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.9 additional information the AaF3H transcripts are accumulated in the cultivar with higher level of flavonoids than that with lower level of flavonoids Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
 Artemisia annua dihydrokaempferol + succinate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.11.9 ? x * 41180, sequence calculation Artemisia annua

Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 AaF3H
-
 Artemisia annua
1.14.11.9 F3H
-
 Artemisia annua
1.14.11.9 flavanone 3-hydroxylase
-
 Artemisia annua

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.9 35
-
-
 Artemisia annua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.9 8.5
-
-
 Artemisia annua

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.11.9 Artemisia annua sequence calculation
-
 5.67