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Literature summary extracted from

  • Pang, Y.; Abeysinghe, I.S.B.; He, J.; He, X.; Huhman, D.
    Mewan, K.M.; Sumner, L.W.; Yun, J.; Dixon, R.A. Functional characterization of proanthocyanidin pathway enzymes from tea and their application for metabolic engineering (2013), Plant Physiol., 161, 1103-1116 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.3 gene LARa, complementary DNA library construction, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, functional expression of His6-tagged enzyme in Escherichia coli strain M15. Recombinant ectopic expression of CsLAR leads to the accumulation of low levels of proanthocyanidin precursors and their conjugates in Medicago truncatula hairy roots and anthocyanin-overproducing Nicotiana tabacum, but levels of oligomeric proanthocyanidins are very low. The expression of CsLAR in tobacco overproducing anthocyanin leads to the accumulation of higher levels of epicatechin and its glucoside than of catechin, phenotype and flavonoid compounds contents, overview Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ Camellia sinensis
-
 (2R,3S)-catechin + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.3 Camellia sinensis I1E425 cv. TRI2043, blister blight-resistant tea cultivar
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.3 leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.3 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+
-
 Camellia sinensis (2R,3S)-catechin + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.3 CsLAR
-
 Camellia sinensis
1.17.1.3 LAR
-
 Camellia sinensis
1.17.1.3 LARa
-
 Camellia sinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.3 NADPH
-
 Camellia sinensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.17.1.3 Camellia sinensis sequence calculation
-
 5.43

General Information

EC Number General Information Comment Organism
1.17.1.3 evolution enzyme LAR is related to members of the reductase-epimerase-dehydrogenase protein superfamily Camellia sinensis