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Literature summary extracted from

  • Kong, W.; Yu, X.; Chen, H.; Liu, L.; Xiao, Y.; Wang, Y.; Wang, C.; Lin, Y.; Yu, Y.; Wang, C.; Jiang, L.; Zhai, H.; Zhao, Z.; Wan, J.
    The catalytic subunit of magnesium-protoporphyrin IX monomethyl ester cyclase forms a chloroplast complex to regulate chlorophyll biosynthesis in rice (2016), Plant Mol. Biol., 92, 177-191 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.81 gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.81 additional information isolation of an Oryza sativa yellow-green leaf 8 (ygl8) mutant that exhibits chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure. Expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. Phenotype, overview. Ygl8 knockout by RNAi Oryza sativa
1.14.13.81 N182S the ygl8 mutation causes a conserved amino acid substitution, which is related to the alterations of chlorophyll precursor content Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.81 chloroplast membrane the YGL8 subunit contains a chloroplast transit peptide Oryza sativa 31969
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 Oryza sativa
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 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.81 Oryza sativa
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.13.81 leaf young, high enzyme content Oryza sativa
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1.14.13.81 additional information subunit YGL8 is constitutively expressed in various tissues, with more abundance in young leaves and panicles Oryza sativa
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1.14.13.81 panicle high enzyme content Oryza sativa
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.81 magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2
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 Oryza sativa 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.81 magnesium-protoporphyrin IX monomethyl ester cyclase
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 Oryza sativa
1.14.13.81 MgPME cyclase
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 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.81 NADPH
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
1.14.13.81 malfunction Oryza sativa yellow-green leaf 8 (ygl8) mutant exhibits a chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure, expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. YGL8 expression demonstrates no differences between the wild-type and ygl8 mutant plants, but the mutant shows increase of Proto IX and MgP/MgPME expression, accompanied with the decrease of protochlorophyllide. Phenotype, overview Oryza sativa
1.14.13.81 additional information protein YGL8 has the dual functions in chlorophyll biosynthesis: one as a catalytic subunit of MgPME cyclase, the other as a core component of FLU-YGL8-LCAA-POR complex in chlorophyll biosynthesis, physical interaction between YGL8 and a rice chloroplast protein, low chlorophyll accumulation A (OsLCAA). YGL8 also interacts with the other two rice chloroplast proteins, viz. fluorescent (OsFLU1) and NADPH:protochlorophyllide oxidoreductase (OsPORB) Oryza sativa