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Literature summary extracted from

  • Mahajan, M.; Yadav, S.K.
    Overexpression of a tea flavanone 3-hydroxylase gene confers tolerance to salt stress and Alternaria solani in transgenic tobacco (2014), Plant Mol. Biol., 85, 551-573 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt Camellia sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 Camellia sinensis
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 Camellia sinensis UPASI-10
-
 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2 Camellia sinensis
-
 dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2 Camellia sinensis UPASI-10
-
 dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2 Camellia sinensis
-
 dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2 Camellia sinensis UPASI-10
-
 dihydrokaempferol + succinate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Camellia sinensis Q6DV45 from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-
1.14.11.9 Camellia sinensis UPASI-10 Q6DV45 from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.9 leaf
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Camellia sinensis a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
 Camellia sinensis UPASI-10 a (2R,3R)-dihydroflavonol + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2
-
 Camellia sinensis dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 eriodictyol + 2-oxoglutarate + O2
-
 Camellia sinensis UPASI-10 dihydroquercetin + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
 Camellia sinensis dihydrokaempferol + succinate + CO2
-
 ?
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
 Camellia sinensis UPASI-10 dihydrokaempferol + succinate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 CsF3H
-
 Camellia sinensis
1.14.11.9 flavanone 3-hydroxylase
-
 Camellia sinensis

General Information

EC Number General Information Comment Organism
1.14.11.9 metabolism flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis Camellia sinensis