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Literature summary extracted from

  • Yahyazadeh, M.; Ratmoyo, P.; Bittner, F.; Sato, F.; Selmar, D.
    Cloning and characterization of Cheilanthifoline and stylopine synthase genes from Chelidonium majus (2017), Plant Cell Physiol., 58, 1421-1430 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.64 heterologously expressed in Saccharomyces cerevisiae Chelidonium majus
1.14.19.65 heterologously expressed in Saccharcomyces cerevisiae Chelidonium majus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.64 (S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2 Chelidonium majus the enzyme is involved in biosynthesis of stylopine (S)-stylopine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.65 (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 Chelidonium majus the enzyme is involved in biosynthesis of stylopine (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.64 Chelidonium majus A0A240FWB4
-
-
1.14.19.65 Chelidonium majus A0A240FWB3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.64 leaf expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different Chelidonium majus
-
1.14.19.64 root expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different Chelidonium majus
-
1.14.19.65 leaf expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different Chelidonium majus
-
1.14.19.65 root expression is very similar in both roots and leaves, although the alkaloid accumulation patterns in these organs are quite different Chelidonium majus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.64 (S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in biosynthesis of stylopine Chelidonium majus (S)-stylopine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.64 (S)-cheilanthifoline + [reduced NADPH-hemoprotein reductase] + O2 high substrate specificity. The only alternative substrate identified is scoulerine, which is converted by stylopine synthase to yield minor amounts of nandinine Chelidonium majus (S)-stylopine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.64 (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2
-
 Chelidonium majus nandinine + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.65 (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 high substrate specificity Chelidonium majus (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.65 (S)-scoulerine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in biosynthesis of stylopine Chelidonium majus (S)-cheilanthifoline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.64 stylopine synthase
-
 Chelidonium majus
1.14.19.65 cheilanthifoline synthase
-
 Chelidonium majus

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.64 NADPH-hemoprotein reductase
-
 Chelidonium majus

General Information

EC Number General Information Comment Organism
1.14.19.64 metabolism the enzyme is involved in biosynthesis of stylopine Chelidonium majus
1.14.19.65 metabolism the enzyme is involved in biosynthesis of stylopine Chelidonium majus