EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.350 | expressed in Escherichia coli Rosetta-gami (DE3) cells | Siraitia grosvenorii |
5.4.99.33 | gene CBS, DNA and amino acid sequence determination and analysis, digital gene expression (DGE) profile analysis, sequence comparisons and phylogenetic analysis of codon-optimized gene, SgCbQ is functionally expressed in the lanosterol synthase-deficient Saccharomyces cerevisiae strain GIL77 leading to accumulation of cucurbitadienol as the sole product | Siraitia grosvenorii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.350 | 0.0414 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 0.0547 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 0.0582 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.350 | UDP-alpha-D-glucose + mogrol | Siraitia grosvenorii | - |
mogroside IE + UDP | - |
? | |
5.4.99.33 | (3S)-2,3-epoxy-2,3-dihydrosqualene | Siraitia grosvenorii | - |
cucurbitadienol | - |
? | |
5.4.99.33 | additional information | Siraitia grosvenorii | GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.350 | Siraitia grosvenorii | - |
- |
- |
5.4.99.33 | Siraitia grosvenorii | K7NBZ9 | cultivated in Guangxi Province of China | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.350 | Ni-NTA agarose column chromatography | Siraitia grosvenorii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.350 | fruit | - |
Siraitia grosvenorii | - |
5.4.99.33 | fruit | expression profiles of SgCbQ gene during the three important stages of fruit development | Siraitia grosvenorii | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.350 | UDP-alpha-D-glucose + mogrol | - |
Siraitia grosvenorii | mogroside IE + UDP | - |
? | |
2.4.1.350 | UDP-alpha-D-glucose + naringenin | - |
Siraitia grosvenorii | ? | - |
? | |
2.4.1.350 | UDP-alpha-D-glucose + quercetin | - |
Siraitia grosvenorii | ? | - |
? | |
5.4.99.33 | (3S)-2,3-epoxy-2,3-dihydrosqualene | - |
Siraitia grosvenorii | cucurbitadienol | - |
? | |
5.4.99.33 | additional information | GC-MS metabolite identification and analysis, extracts from Saccharomyces cerevisiae transformants expressing enzyme SgCbQ produce only one new peak, that is identified as cucurbitadienol | Siraitia grosvenorii | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.350 | ? | x * 52000, SDS-PAGE | Siraitia grosvenorii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.350 | UGT74AC1 | - |
Siraitia grosvenorii |
5.4.99.33 | CBS | - |
Siraitia grosvenorii |
5.4.99.33 | SgCbQ | - |
Siraitia grosvenorii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.350 | 0.000411 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 0.41 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 0.45 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii |
EC Number | General Information | Comment | Organism |
---|---|---|---|
5.4.99.33 | evolution | SgCbQ clusters with the other three functionally characterized cucurbitadienol synthases and forms a new subfamily that lies between the branches of cycloartenol synthase and lanosterol synthase, non-rooted phylogenetic tree analysis, overview | Siraitia grosvenorii |
5.4.99.33 | metabolism | cucurbitadienol synthase and triterpene glycosyltransferase are involved in biosynthesis of mogrosides in Siraitia grosvenorii. Mogrosides, the major bioactive components isolated from the fruits of Siraitia grosvenorii, are a family of cucurbitane-type tetracyclic triterpenoid saponins that are used worldwide as high-potency sweeteners and possess a variety of notable pharmacological activities. Mogrosides are synthesized from 2,3-oxidosqualene via a series of reactions catalyzed by cucurbitadienol synthase (CbQ), Cyt P450s (P450s), and UDP glycosyltransferases (UGTs) in vivo, proposed biosynthetic pathway of mogrosides, overview | Siraitia grosvenorii |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.350 | 0.00994 | - |
mogrol | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 6.88 | - |
quercetin | at pH 7.2 and 30°C | Siraitia grosvenorii | |
2.4.1.350 | 8.23 | - |
naringenin | at pH 7.2 and 30°C | Siraitia grosvenorii |