BRENDA - Enzyme Database

Gallocatechin biosynthesis via a flavonoid 3',5'-hydroxylase is a defense response in Norway spruce against infection by the bark beetle-associated sap-staining fungus Endoconidiophora polonica

Hammerbacher, A.; Raguschke, B.; Wright, L.; Gershenzon, J.; Phytochemistry 148, 78-86 (2018)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.81
2,3-(trans)-(+)-catechin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
2,3-(trans)-(+)-gallocatechin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.81
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.81
leucocyanidin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
leucodelphinidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.81
Picea abies
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.81
2,3-(trans)-(+)-catechin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
2,3-(trans)-(+)-gallocatechin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.81
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.81
leucocyanidin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
leucodelphinidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.81
2,3-(trans)-(+)-catechin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
2,3-(trans)-(+)-gallocatechin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.81
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
1.14.14.81
leucocyanidin + [reduced NADPH-hemoprotein reductase] + O2
Picea abies
-
leucodelphinidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.81
2,3-(trans)-(+)-catechin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
2,3-(trans)-(+)-gallocatechin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.81
dihydroquercetin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
dihydromyricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
1.14.14.81
leucocyanidin + [reduced NADPH-hemoprotein reductase] + O2
-
746005
Picea abies
leucodelphinidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.14.81
Picea abies
transcript abundance of the flavonoid 3',5'-hydroxylase gene increases significantly during infection with fungus Endoconidiophora polonica
up
General Information
EC Number
General Information
Commentary
Organism
1.14.14.81
physiological function
coexpression with Norway spruce and apple genes encoding the rest of the flavonoid pathway, PaCHS, PaF3H, PaF30H, MdDFR and PaLAR3, in Nicotiana benthamiana leaves. Catechin and 2,3-(trans)-(+)-gallocatechin accumulate in the leaves 7 days after Agrobacterium infiltration. There is a significant increase in the gallocatechin content of Norway spruce bark and wood after inoculation with the bark beetle-associated sap-staining fungus Endoconidiophora polonica. 2,3 (trans)-(+)-catechin is a stronger inhibitor of fungal growth, while 2,3 (trans)-(+)-gallocatechin is a stronger inhibitor of melanin biosynthesis
Picea abies
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.14.81
physiological function
coexpression with Norway spruce and apple genes encoding the rest of the flavonoid pathway, PaCHS, PaF3H, PaF30H, MdDFR and PaLAR3, in Nicotiana benthamiana leaves. Catechin and 2,3-(trans)-(+)-gallocatechin accumulate in the leaves 7 days after Agrobacterium infiltration. There is a significant increase in the gallocatechin content of Norway spruce bark and wood after inoculation with the bark beetle-associated sap-staining fungus Endoconidiophora polonica. 2,3 (trans)-(+)-catechin is a stronger inhibitor of fungal growth, while 2,3 (trans)-(+)-gallocatechin is a stronger inhibitor of melanin biosynthesis
Picea abies
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.14.81
Picea abies
transcript abundance of the flavonoid 3',5'-hydroxylase gene increases significantly during infection with fungus Endoconidiophora polonica
up