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Literature summary extracted from

  • Ibdah, M.; Berim, A.; Martens, S.; Valderrama, A.L.; Palmieri, L.; Lewinsohn, E.; Gang, D.R.
    Identification and cloning of an NADPH-dependent hydroxycinnamoyl-CoA double bond reductase involved in dihydrochalcone formation in Malus x domestica Borkh (2014), Phytochemistry, 107, 24-31 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.117 expression in Escherichia coli Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.117 0.093
-
 trans-feruloyl-CoA pH 50, 35°C Malus domestica
1.3.1.117 0.097
-
 trans-4-coumaroyl-CoA pH 50, 35°C Malus domestica
1.3.1.117 0.101
-
 NADPH pH 50, 35°C Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.117 trans-4-coumaroyl-CoA + NADPH + H+ Malus domestica enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 dihydro-4-coumaroyl-CoA + NADP+
-
 ?
1.3.1.117 trans-feruloyl-CoA + NADPH + H+ Malus domestica enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 dihydroferuloyl-CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.117 Malus domestica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.117 trans-4-coumaroyl-CoA + NADPH + H+
-
 Malus domestica dihydro-4-coumaroyl-CoA + NADP+
-
 ?
1.3.1.117 trans-4-coumaroyl-CoA + NADPH + H+ enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 Malus domestica dihydro-4-coumaroyl-CoA + NADP+
-
 ?
1.3.1.117 trans-feruloyl-CoA + NADPH + H+
-
 Malus domestica dihydroferuloyl-CoA + NADP+
-
 ?
1.3.1.117 trans-feruloyl-CoA + NADPH + H+ enzyme prefers feruloyl-CoA to p-coumaroyl-CoA by a factor of 2.1 Malus domestica dihydroferuloyl-CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.117 ? x * 38400, SDS-PAGE and calculated from sequence Malus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.117 40
-
-
 Malus domestica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.117 0.0019
-
 trans-4-coumaroyl-CoA pH 50, 35°C Malus domestica
1.3.1.117 0.0023
-
 NADPH pH 50, 35°C Malus domestica
1.3.1.117 0.0042
-
 trans-feruloyl-CoA pH 50, 35°C Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.117 NADP+
-
 Malus domestica
1.3.1.117 NADPH
-
 Malus domestica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.1.117 Malus domestica calculated from sequence
-
 6.9