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Literature summary extracted from

  • Tian, Q.; Wang, C.; Liu, Y.; Xie, W.
    Structural basis for recognition of G-1-containing tRNA by histidyl-tRNA synthetase (2015), Nucleic Acids Res., 43, 2980-2990 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.21 expressed in Escherichia coli BL21(DE3) cells Thermus thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.21 enzyme complexed with tRNAHis, L-histidine, and AMPPNP, hanging drop vapor diffusion method, using 5% (w/v) PEG 4000 and 0.1 M NaOAc (pH4.5) Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.21 325-328Del the mutant shows about 20% of wild type activity Thermus thermophilus
6.1.1.21 328insTTET the mutant shows about 80% of wild type activity Thermus thermophilus
6.1.1.21 E114A inactive Thermus thermophilus
6.1.1.21 E388A the mutant shows about 15% of wild type activity Thermus thermophilus
6.1.1.21 E64A inactive Thermus thermophilus
6.1.1.21 G285A inactive Thermus thermophilus
6.1.1.21 G286A the mutant shows about 2% of wild type activity Thermus thermophilus
6.1.1.21 G72V the mutant shows about 50% of wild type activity Thermus thermophilus
6.1.1.21 G72V/G73V the mutant shows about 15% of wild type activity Thermus thermophilus
6.1.1.21 K209A the mutant shows about 80% of wild type activity Thermus thermophilus
6.1.1.21 K397Q the mutant shows about 10% of wild type activity Thermus thermophilus
6.1.1.21 Q404A the mutant shows about 90% of wild type activity Thermus thermophilus
6.1.1.21 R115A the mutant shows about 20% of wild type activity Thermus thermophilus
6.1.1.21 R120A the mutant shows about 8% of wild type activity Thermus thermophilus
6.1.1.21 R122A the mutant shows about 15% of wild type activity Thermus thermophilus
6.1.1.21 R122K the mutant shows about 55% of wild type activity Thermus thermophilus
6.1.1.21 R197A the mutant shows about 50% of wild type activity Thermus thermophilus
6.1.1.21 R7A the mutant shows about 20% of wild type activity Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis Thermus thermophilus
-
 AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Thermus thermophilus P62374
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.21 Ni-NTA column chromatography and Superdex 200 gel filtration Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Thermus thermophilus AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.21 HisRS
-
 Thermus thermophilus
6.1.1.21 Histidyl-tRNA synthetase
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.21 ATP
-
 Thermus thermophilus