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Literature summary extracted from

  • Lubner, C.E.; Jennings, D.P.; Mulder, D.W.; Schut, G.J.; Zadvornyy, O.A.; Hoben, J.P.; Tokmina-Lukaszewska, M.; Berry, L.; Nguyen, D.M.; Lipscomb, G.L.; Bothner, B.; Jones, A.K.; Miller, A.F.; King, P.W.; Adams, M.W.W.; Peters, J.W.
    Mechanistic insights into energy conservation by flavin-based electron bifurcation (2017), Nat. Chem. Biol., 13, 655-659 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.1.4
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 Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
1.6.1.4 Pyrococcus furiosus Q8U195 and Q8U194 Q8U195: subunit alpha, Q8U194: subunit beta
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.1.4
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.1.4 NAD+ + 2 NADPH + 2 oxidized ferredoxin [iron-sulfur] cluster reduction of both NAD+ and Fd by Nfn enzymes is coupled to the bifurcation of electrons from NADPH oxidation. The exergonic coupling of the NADP(H) and NAD(H) half reactions in Nfn is presumed to be mediated by NADPH oxidation at L-FAD followed by electron transfer to the proximal [2Fe-2S] cluster and subsequently the NAD(H) binding site at S-FAD. Biophysical analysis of the enzyme provides the first direct insight into the mechanism of flavin-based electron bifurcation and the requisite structural features Pyrococcus furiosus NADH + H+ + 2 NADP+ + 2 reduced ferredoxin [iron-sulfur] cluster
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 ?

Subunits

EC Number Subunits Comment Organism
1.6.1.4 heterodimer 1 * 31000 + 1 * 53000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
1.6.1.4 NfnI
-
 Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.1.4 FAD the 31000 Da subunit contains one FAD (S-FAD) and a [2Fe-2S] cluster with an unusual Asp ligand. The 53000 Da subunit contains one FAD (L-FAD), which is the site of electron bifurcation, and two [4Fe-4S] clusters. The L-FAD proximal [4Fe-4S] cluster coordination includes an unusual Glu ligand. S-FAD and L-FAD bind NADH and NADPH, respectively Pyrococcus furiosus
1.6.1.4 [2Fe-2S]-center the 31000 Da subunit contains one FAD (S-FAD) and a [2Fe-2S] cluster. The [2Fe-2S] cluster is unusual, with a relatively high (positive) reduction potential and coordination by one Asp and three Cys ligands Pyrococcus furiosus
1.6.1.4 [4Fe-4S]-center the 53000 Da subunit contains one FAD (L-FAD), which is the site of electron bifurcation, and two [4Fe-4S] clusters Pyrococcus furiosus