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Literature summary extracted from

  • Mestre, P.; Arista, G.; Piron, M.C.; Rustenholz, C.; Ritzenthaler, C.; Merdinoglu, D.; Chich, J.F.
    Identification of a Vitis vinifera endo-beta-1,3-glucanase with antimicrobial activity against Plasmopara viticola (2017), Mol. Plant Pathol., 18, 708-719 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.39 agriculture isoform EGase3 shows strong antimicrobial activity against germinated spores of Plasmopara viticola Vitis vinifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.39 expression in Escherichia coli Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.39 0.081
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 0.147
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 0.228
-
 Laminarin pH 5.4, 37°C Vitis vinifera

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.39 Vitis vinifera A7PQW3 isoform EGase2
-
3.2.1.39 Vitis vinifera F6HLL8 isoform EGase3
-
3.2.1.39 Vitis vinifera Q9M563 isoform EGase1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.39 laminarin + H2O
-
 Vitis vinifera ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.39 ? x * 37000, SDS-PAGE, x * 37100, calculated Vitis vinifera
3.2.1.39 ? x * 39000, SDS-PAGE, x * 38500, calculated Vitis vinifera

Synonyms

EC Number Synonyms Comment Organism
3.2.1.39 EGase1
-
 Vitis vinifera
3.2.1.39 EGase2
-
 Vitis vinifera
3.2.1.39 EGase3
-
 Vitis vinifera
3.2.1.39 VIT_205s0077g01150
-
 Vitis vinifera
3.2.1.39 VIT_206s0061g00120
-
 Vitis vinifera
3.2.1.39 VIT_208s0007g06060
-
 Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.39 40
-
-
 Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.39 3
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 34
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 92
-
 Laminarin pH 5.4, 37°C Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.39 5
-
-
 Vitis vinifera
3.2.1.39 5.5
-
-
 Vitis vinifera

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.39 Vitis vinifera calculated
-
 4.9
3.2.1.39 Vitis vinifera calculated
-
 9.4
3.2.1.39 Vitis vinifera calculated
-
 9.5

Expression

EC Number Organism Comment Expression
3.2.1.39 Vitis vinifera expression is induced by Plasmapora viticola infection up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.39 40
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 230
-
 Laminarin pH 5.4, 37°C Vitis vinifera
3.2.1.39 400
-
 Laminarin pH 5.4, 37°C Vitis vinifera