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Literature summary extracted from

  • Shrestha, P.; Yun, J.H.; Kim, W.T.; Kim, T.Y.; Lee, W.
    Cloning, purification, and characterization of recombinant human extracellular superoxide dismutase in SF9 insect cells (2016), Mol. Cells, 39, 242-249 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.15.1.1 medicine SOD3 has the potential as a therapeutic drug for various diseases Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 gene SOD3, cloning of EC-SOD and recombinant expression of wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method, both full length and truncated hEC-SOD proteins are enzymatically active Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.15.1.1 additional information generation of a catalytically active truncated (residues 19-240) mutant form of human EC-SOD (hEC-SOD) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 diethyldithiocarbamate
-
Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.15.1.1 extracellular
-
Homo sapiens
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ required, enzyme-bound Homo sapiens
1.15.1.1 additional information added Zn2+ and Cu2+ do not affect the enzyme activity or structure Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Homo sapiens
-
O2 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Homo sapiens P08294
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.15.1.1 glycoprotein recombinant hEC-SOD expressed in Sf9 insect cells is glycosylated at Asn107. The glycosylated form of hEC-SOD is soluble, whereas the unglycosylated form is not, but it can be solubilized by increasing the pH and ionic strength of the solution Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes from Spodoptera frugiperda Sf9 cells by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
Homo sapiens O2 + H2O2
-
?
1.15.1.1 additional information enzyme activity detection by water-soluble tetrazolium (WST-1) assay. This assay is based on the detection of a water-soluble formazan dye that is formed upon reduction of water-soluble tetrazolium salt, WST-1, by the superoxide anion Homo sapiens ?
-
?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 dimer 2 * 33000, recombinant enzyme, SDS-PAGE Homo sapiens
1.15.1.1 monomer 1 * 33000, recombinant enzyme, SDS-PAGE Homo sapiens
1.15.1.1 More hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), enzyme secondary structure determination by circular dichroism, recombinant hEC-SOD purified from Sf9 insect cells is mainly composed of beta-sheet structures Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 EC-SOD
-
Homo sapiens
1.15.1.1 extracellular superoxide dismutase
-
Homo sapiens
1.15.1.1 hEC-SOD
-
Homo sapiens
1.15.1.1 SOD3
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.4 9 assay at Homo sapiens