EC Number | Application | Comment | Organism |
---|---|---|---|
1.15.1.1 | medicine | SOD3 has the potential as a therapeutic drug for various diseases | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.15.1.1 | gene SOD3, cloning of EC-SOD and recombinant expression of wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method, both full length and truncated hEC-SOD proteins are enzymatically active | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.15.1.1 | additional information | generation of a catalytically active truncated (residues 19-240) mutant form of human EC-SOD (hEC-SOD) | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | diethyldithiocarbamate | - |
Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.15.1.1 | extracellular | - |
Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.15.1.1 | Cu2+ | required, enzyme-bound | Homo sapiens | |
1.15.1.1 | additional information | added Zn2+ and Cu2+ do not affect the enzyme activity or structure | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ | Homo sapiens | - |
O2 + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.15.1.1 | Homo sapiens | P08294 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.15.1.1 | glycoprotein | recombinant hEC-SOD expressed in Sf9 insect cells is glycosylated at Asn107. The glycosylated form of hEC-SOD is soluble, whereas the unglycosylated form is not, but it can be solubilized by increasing the pH and ionic strength of the solution | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.15.1.1 | recombinant wild-type and truncated mutant FLAG-tagged hEC-SOD enzymes from Spodoptera frugiperda Sf9 cells by affinity chromatography | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.15.1.1 | 2 superoxide + 2 H+ | - |
Homo sapiens | O2 + H2O2 | - |
? | |
1.15.1.1 | additional information | enzyme activity detection by water-soluble tetrazolium (WST-1) assay. This assay is based on the detection of a water-soluble formazan dye that is formed upon reduction of water-soluble tetrazolium salt, WST-1, by the superoxide anion | Homo sapiens | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.15.1.1 | dimer | 2 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
1.15.1.1 | monomer | 1 * 33000, recombinant enzyme, SDS-PAGE | Homo sapiens |
1.15.1.1 | More | hEC-SOD is present both as a monomer (33 kDa) and a dimer (66 kDa), enzyme secondary structure determination by circular dichroism, recombinant hEC-SOD purified from Sf9 insect cells is mainly composed of beta-sheet structures | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.15.1.1 | EC-SOD | - |
Homo sapiens |
1.15.1.1 | extracellular superoxide dismutase | - |
Homo sapiens |
1.15.1.1 | hEC-SOD | - |
Homo sapiens |
1.15.1.1 | SOD3 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.15.1.1 | 7.4 | 9 | assay at | Homo sapiens |