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Literature summary extracted from

  • van Vugt-Lussenburg, B.; van der Weel, L.; Hagen, W.; Hagedoorn, P.
    Identification of two [4Fe-4S]-cluster-containing hydro-lyases from Pyrococcus furiosus (2009), Microbiology, 155, 3015-3020 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.2 overexpression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.34
-
fumarate pH 8, 75°C Pyrococcus furiosus
4.2.1.2 0.41
-
(S)-malate pH 8, 75°C Pyrococcus furiosus
4.2.1.2 0.51
-
D-Tartrate pH 8, 75°C Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.2 Iron [4Fe-4S]-cluster containing heterodimeric enzyme Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Pyrococcus furiosus Q8U062 and Q8U063 Q8U062: alpha-subunit, Q8U063: beta-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
Pyrococcus furiosus fumarate + H2O
-
r
4.2.1.2 D-tartrate
-
Pyrococcus furiosus ?
-
?
4.2.1.2 fumarate + H2O
-
Pyrococcus furiosus (S)-malate
-
r

Subunits

EC Number Subunits Comment Organism
4.2.1.2 heterodimer
-
Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 PF1754 gene name, PF1755 and PF1754 were shown to form a [4Fe-4S]-clustercontaining heterodimeric enzyme, able to catalyse the reversible hydratation of fumarate Pyrococcus furiosus
4.2.1.2 PF1755 gene name, PF1755 and PF1754 were shown to form a [4Fe-4S]-clustercontaining heterodimeric enzyme, able to catalyse the reversible hydratation of fumarate Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.2 75
-
assay at Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8
-
assay at Pyrococcus furiosus

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.2 [4Fe-4S]-center [4Fe-4S]-cluster containing heterodimeric enzyme Pyrococcus furiosus

General Information

EC Number General Information Comment Organism
4.2.1.2 metabolism the enzyme probably plays a role in amino acid synthesis when the organism grows on carbohydrates Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.2 930
-
D-Tartrate pH 8, 75°C Pyrococcus furiosus
4.2.1.2 3200
-
fumarate pH 8, 75°C Pyrococcus furiosus
4.2.1.2 3700
-
(S)-malate pH 8, 75°C Pyrococcus furiosus