EC Number | Cloned (Comment) | Organism |
---|---|---|
6.3.1.2 | gene LdGS, the enzyme exists as a single copy gene in parasite genome, sequence comparisons and phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Leishmania donovani |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | L-methionine sulfone | - |
Leishmania donovani | |
6.3.1.2 | L-methionine sulfoximine | - |
Leishmania donovani | |
6.3.1.2 | Phosphinothricin | - |
Leishmania donovani |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 26.3 | - |
L-glutamate | recombinant enzyme, pH 7.8, 37°C | Leishmania donovani |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.1.2 | cytoplasm | - |
Leishmania donovani | 5737 | - |
6.3.1.2 | additional information | presence of mitochondrial targeting sequence and the cleavage localization signal at the N-terminal region in LdGS protein | Leishmania donovani | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | Ca2+ | can partially substitute for Mg2+ | Leishmania donovani | |
6.3.1.2 | Mg2+ | required | Leishmania donovani | |
6.3.1.2 | Mn2+ | can partially substitute for Mg2+ | Leishmania donovani | |
6.3.1.2 | Ni2+ | can partially substitute for Mg2+ | Leishmania donovani | |
6.3.1.2 | Zn2+ | can partially substitute for Mg2+ | Leishmania donovani |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-glutamate + NH3 | Leishmania donovani | - |
ADP + phosphate + L-glutamine | - |
r | |
6.3.1.2 | ATP + L-glutamate + NH3 | Leishmania donovani BPK282A1 | - |
ADP + phosphate + L-glutamine | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Leishmania donovani | E9B8P8 | MHOM/80/IN/Dd8 | - |
6.3.1.2 | Leishmania donovani BPK282A1 | E9B8P8 | MHOM/80/IN/Dd8 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.1.2 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Leishmania donovani |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
6.3.1.2 | amastigote | - |
Leishmania donovani | - |
6.3.1.2 | promastigote | - |
Leishmania donovani | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 0.322 | - |
enzyme from promastigotes, pH 7.8, 37°C | Leishmania donovani |
6.3.1.2 | 0.7325 | - |
recombinant His-tagged enzyme, pH 7.8, 37°C | Leishmania donovani |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-glutamate + NH3 | - |
Leishmania donovani | ADP + phosphate + L-glutamine | - |
r | |
6.3.1.2 | ATP + L-glutamate + NH3 | - |
Leishmania donovani BPK282A1 | ADP + phosphate + L-glutamine | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.2 | ? | x * 43500, recombinant His-tagged enzyme, SDS-PAGE | Leishmania donovani |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.1.2 | LDBPK_060370 | - |
Leishmania donovani |
6.3.1.2 | LdGS | - |
Leishmania donovani |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 37 | - |
assay at | Leishmania donovani |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 8 | - |
- |
Leishmania donovani |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 6 | 11 | activity range | Leishmania donovani |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | ATP | - |
Leishmania donovani |