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Literature summary extracted from
- Application of a delta-6 desaturase with alpha-linolenic acid preference on eicosapentaenoic acid production in Mortierella alpina (2016), Microb. Cell Fact., 15, 117 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.19.3 | recombinant overexpression of MpFADS6 in a uracil-auxotrophic Mortierella alpina strain to enhance eicosapentaenoic acid production in Mortierella alpina by favoring the omega3 pathway, using the Agrobacterium tumefaciens-mediated transformation method. The expression of MpFADS6 results in a 26.2fold increase in EPA production compared to wild-type Mortierella alpina | Micromonas pusilla |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.19.3 | additional information | recombinant overexpression of Micromonas pusilla strain CCMP 1545 enzyme MpFADS6 in a uracil-auxotrophic Mortierella alpina strain to enhance eicosapentaenoic acid production in Mortierella alpina by favoring the omega3 pathway, using the Agrobacterium tumefaciens-mediated transformation method. The expression of MpFADS6 results in a 26.2fold increase in EPA production compared to wild-type Mortierella alpina, fatty acid profile with addition of exogenous alpha-linolenoic acid. Further enhancement of EPA production with peony seed meal, overview | Mortierella alpina |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.19.3 | membrane | - |
Mortierella alpina | 16020 | - |
| 1.14.19.3 | membrane | - |
Micromonas pusilla | 16020 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Mortierella alpina | - |
stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? |  |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Micromonas pusilla | preferred substrate | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Mortierella alpina ATCC 32222 | - |
stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Micromonas pusilla CCMP 1545 | preferred substrate | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Micromonas pusilla | - |
gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Mortierella alpina | preferred substrate | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Mortierella alpina ATCC 32222 | preferred substrate | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | Micromonas pusilla CCMP 1545 | - |
gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | additional information | Mortierella alpina | polyunsaturated fatty acid profiles in Mortierella alpina, the lipids are synthesized mainly via the omega6 pathway and rarely via the omega3 pathway and as a result contain low alpha-linolenoyl-CoA and eicosapentaenoic acid levels | ? | - |
? | |
| 1.14.19.3 | additional information | Mortierella alpina ATCC 32222 | polyunsaturated fatty acid profiles in Mortierella alpina, the lipids are synthesized mainly via the omega6 pathway and rarely via the omega3 pathway and as a result contain low alpha-linolenoyl-CoA and eicosapentaenoic acid levels | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.19.3 | Micromonas pusilla | C1MKR5 | - |
- |
| 1.14.19.3 | Micromonas pusilla CCMP 1545 | C1MKR5 | - |
- |
| 1.14.19.3 | Mortierella alpina | Q70BL2 | - |
- |
| 1.14.19.3 | Mortierella alpina ATCC 32222 | Q70BL2 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.19.3 | additional information | growth temperatures 12-28°C | Mortierella alpina | - |
| 1.14.19.3 | mycelium | - |
Mortierella alpina | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Mortierella alpina | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | preferred substrate | Micromonas pusilla | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Mortierella alpina ATCC 32222 | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | preferred substrate | Micromonas pusilla CCMP 1545 | stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Micromonas pusilla | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | preferred substrate | Mortierella alpina | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | preferred substrate | Mortierella alpina ATCC 32222 | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | linoleoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Micromonas pusilla CCMP 1545 | gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H2O | - |
? | |
| 1.14.19.3 | additional information | polyunsaturated fatty acid profiles in Mortierella alpina, the lipids are synthesized mainly via the omega6 pathway and rarely via the omega3 pathway and as a result contain low alpha-linolenoyl-CoA and eicosapentaenoic acid levels | Mortierella alpina | ? | - |
? | |
| 1.14.19.3 | additional information | polyunsaturated fatty acid profiles in Mortierella alpina, the lipids are synthesized mainly via the omega6 pathway and rarely via the omega3 pathway and as a result contain low alpha-linolenoyl-CoA and eicosapentaenoic acid levels | Mortierella alpina ATCC 32222 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.19.3 | DELTA-6 desaturase | - |
Mortierella alpina |
| 1.14.19.3 | DELTA-6 desaturase | - |
Micromonas pusilla |
| 1.14.19.3 | delta61 | - |
Mortierella alpina |
| 1.14.19.3 | FADS6 | - |
Mortierella alpina |
| 1.14.19.3 | FADS6 | - |
Micromonas pusilla |
| 1.14.19.3 | MaFADS6 | - |
Mortierella alpina |
| 1.14.19.3 | MaFADS6-I | - |
Mortierella alpina |
| 1.14.19.3 | MpFADS6 | - |
Micromonas pusilla |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.19.3 | cytochrome b5 | - |
Mortierella alpina | |
| 1.14.19.3 | cytochrome b5 | - |
Micromonas pusilla |
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Release 2023.1 (January 2023)
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