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Literature summary extracted from

  • Yuan, M.; Zhang, Y.; Zhao, L.; Ma, Y.; He, Q.; He, J.; Qiu, J.
    Identification and characterization of a new three-component nicotinic acid hydroxylase NahAB1 B2 from Pusillimonas sp. strain T2 (2018), Lett. Appl. Microbiol., 66, 321-328 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.17.1.5 2,6-dichloroindophenol after addition of an artificial electron acceptor such as 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 phenazine methosulfate after addition of an artificial electron acceptor such as phenazine methosulfate, PMS, the nicotinate hydroxylase activity is significantly increased Pusillimonas sp. (ex Stolz et al. 2005)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.5 genes nahAB1B2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant enzyme expression in the non-nicotinic acid-degrading Shinella sp. strain HZN7. The recombinant HZN7-pBBR-nahAB1B2 converts nicotinic acid into equimolar 6-hydroxynicotinic acid, while the recombinants HZN7-pBBR-nahAB1 (lacking component B2) and HZN7-pBBRnahAB2 (lacking component B1) cannot convert nicotinic acid. Cell-free extracts of HZN7-pBBR-nahAB1B2 exhibit nicotinic acid hydroxylase activity Pusillimonas sp. (ex Stolz et al. 2005)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.5 0.0659
-
 nicotinate recombinant enzyme, with phenazine methosulfate, pH 7.0, 30°C Pusillimonas sp. (ex Stolz et al. 2005)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.5 nicotinate + H2O + NAD+ Pusillimonas sp. (ex Stolz et al. 2005)
-
 6-hydroxynicotinate + NADH + H+
-
 ?
1.17.1.5 nicotinate + H2O + NAD+ Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
 6-hydroxynicotinate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.5 Pusillimonas sp. (ex Stolz et al. 2005)
-
-
-
1.17.1.5 Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.1.5 cell culture strain T2 is capable of utilizing nicotinic acid as sole carbon and nitrogen source Pusillimonas sp. (ex Stolz et al. 2005)
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.5 additional information after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased Pusillimonas sp. (ex Stolz et al. 2005) ?
-
 ?
1.17.1.5 additional information after addition of an artificial electron acceptor, such as phenazine methosulfate, or 2,6-dichloroindophenol, the nicotinate hydroxylase activity is significantly increased Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 ?
-
 ?
1.17.1.5 nicotinate + H2O + 2,6-dichloroindophenol
-
 Pusillimonas sp. (ex Stolz et al. 2005) 6-hydroxynicotinate + reduced 2,6-dichloroindophenol
-
 ?
1.17.1.5 nicotinate + H2O + 2,6-dichloroindophenol
-
 Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 6-hydroxynicotinate + reduced 2,6-dichloroindophenol
-
 ?
1.17.1.5 nicotinate + H2O + NAD+
-
 Pusillimonas sp. (ex Stolz et al. 2005) 6-hydroxynicotinate + NADH + H+
-
 ?
1.17.1.5 nicotinate + H2O + NAD+
-
 Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 6-hydroxynicotinate + NADH + H+
-
 ?
1.17.1.5 nicotinate + H2O + phenazine methosulfate
-
 Pusillimonas sp. (ex Stolz et al. 2005) 6-hydroxynicotinate + reduced phenazine methosulfate
-
 ?
1.17.1.5 nicotinate + H2O + phenazine methosulfate
-
 Pusillimonas sp. (ex Stolz et al. 2005) T2 / CCTCC M2014272 6-hydroxynicotinate + reduced phenazine methosulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.5 NA hydroxylase
-
 Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 NahAB1B2
-
 Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 nicotinic acid hydroxylase
-
 Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 three-component NA hydroxylase
-
 Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 three-component nicotinic acid hydroxylase
-
 Pusillimonas sp. (ex Stolz et al. 2005)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.5 30
-
 assay at Pusillimonas sp. (ex Stolz et al. 2005)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.5 7
-
 assay at Pusillimonas sp. (ex Stolz et al. 2005)

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.5 FAD flavoenzyme Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 additional information no activity with FMN and NADP+ Pusillimonas sp. (ex Stolz et al. 2005)
1.17.1.5 NAD+
-
 Pusillimonas sp. (ex Stolz et al. 2005)

General Information

EC Number General Information Comment Organism
1.17.1.5 physiological function the enzyme is involved in the degradation of nicotinic acid Pusillimonas sp. (ex Stolz et al. 2005)