Any feedback?
Literature summary extracted from
- Hydroxylation of methane through component interactions in soluble methane monooxygenases (2016), J. Microbiol., 54, 277-282 .
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.25 | cytoplasm | - |
Methylococcus capsulatus | 5737 | - |
| 1.14.13.25 | cytoplasm | - |
Methylosinus trichosporium | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.25 | Fe3+ | the enzyme has diiron (FeIII-FeIII) active sites where different types of hydrocarbons are oxidized through orchestrated hydroxylase, regulatory and reductase components for precise control of hydrocarbons, oxygen, protons, and electrons | Methylococcus capsulatus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.25 | 251000 | - |
- |
Methylococcus capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | Methylococcus capsulatus | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | Methylosinus trichosporium | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | Methylococcus capsulatus Bath. | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | methanol + NAD(P)+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.25 | Methylococcus capsulatus | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
| 1.14.13.25 | Methylococcus capsulatus Bath. | P22869 and P18798 and P11987 and P18797 and P22868 and P22867 | P22869 (MmoX), P18798 (MmoY), P11987 (MmoZ), P18797 (MmoB), P22868 (MmoC), P22867 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
| 1.14.13.25 | Methylosinus trichosporium | P27353 and P27355 and P27354 and P27356 and Q53563 and Q53562 | P27353 (alpha/MmoX), P27355 (gamma/MmoZ), P27354 (beta/MmoY), P27356 (MmoB), Q53563 (MmoC), Q53562 (MmoD). The soluble methane monooxygenase (sMMO) consists of four components A/MMOH (composed of alpha/MmoX, beta/MmoY and gamma/MmoZ), B/MMOB (MmoB), C/MMOR (MmoC) and D/MMOD (MmoD) | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | - |
Methylococcus capsulatus Bath. | methanol + NAD(P)+ + H2O | - |
? | |
| 1.14.13.25 | methane + NAD(P)H + H+ + O2 | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus Bath. | methanol + NAD(P)+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | ? | the hydroxylase (MMOH) component is a homodimer that consists of two protomers, and each protomer has three polypeptides (alphabetagamma), 60600 (alpha/MmoX) + 40500 (beta/MmoY) + 19800 (gamma/MmoZ), two equivalent of the regulatory component (MmoB) bind to one equivalent MMOH (alpha2beta2gamma2), + reductase protein (MmoC), + 12000 (MmoD) | Methylococcus capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | sMMO | - |
Methylococcus capsulatus |
| 1.14.13.25 | sMMO | - |
Methylosinus trichosporium |
| 1.14.13.25 | soluble methane monooxygenase | - |
Methylosinus trichosporium |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.13.25 | Methylococcus capsulatus | expressed in copper-limited conditions | up |
| 1.14.13.25 | Methylosinus trichosporium | expressed in copper-limited conditions | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.13.25 | metabolism | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylococcus capsulatus |
| 1.14.13.25 | metabolism | methane hydroxylation through methane monooxygenases is a key aspect due to their control of the carbon cycle in the ecology system | Methylosinus trichosporium |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
