BRENDA - Enzyme Database

Methyl farnesoate epoxidase (mfe) gene expression and juvenile hormone titers in the life cycle of a highly eusocial stingless bee, Melipona scutellaris

Cardoso-Junior, C.A.M.; Silva, R.P.; Borges, N.A.; de Carvalho, W.J.; Walter, S.L.; Simoes, Z.L.P.; Bitondi, M.M.G.; Ueira Vieira, C.; Bonetti, A.M.; Hartfelder, K.; J. Insect Physiol. 101, 185-194 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.13.202
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
Melipona scutellaris
1.14.14.127
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
Melipona scutellaris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
Melipona scutellaris
-
juvenile hormone III + NADP+ + H2O
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Melipona scutellaris
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.13.202
Melipona scutellaris
A0A286LD89
collected from colonies kept in the Meliponary of the University of Uberlandia, MG Brazil
-
1.14.14.127
Melipona scutellaris
A0A286LD89
collected from colonies kept in the Meliponary of the University of Uberlandia, MG Brazil
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.202
adult
-
Melipona scutellaris
-
1.14.13.202
corpus allatum
gene mfe is exclusively expressed in the corpora allata
Melipona scutellaris
-
1.14.13.202
larva
-
Melipona scutellaris
-
1.14.13.202
additional information
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
Melipona scutellaris
-
1.14.13.202
pupa
-
Melipona scutellaris
-
1.14.14.127
adult
-
Melipona scutellaris
-
1.14.14.127
corpus allatum
gene mfe is exclusively expressed in the corpora allata
Melipona scutellaris
-
1.14.14.127
larva
-
Melipona scutellaris
-
1.14.14.127
additional information
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
Melipona scutellaris
-
1.14.14.127
pupa
-
Melipona scutellaris
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
-
745508
Melipona scutellaris
juvenile hormone III + NADP+ + H2O
-
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
745508
Melipona scutellaris
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.13.202
methylfarnesoate epoxidase
-
Melipona scutellaris
1.14.13.202
MFE
-
Melipona scutellaris
1.14.14.127
methylfarnesoate epoxidase
-
Melipona scutellaris
1.14.14.127
MFE
-
Melipona scutellaris
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.202
cytochrome P450
-
Melipona scutellaris
1.14.13.202
NADPH
-
Melipona scutellaris
1.14.14.127
cytochrome P450
-
Melipona scutellaris
1.14.14.127
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Melipona scutellaris
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.202
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
Melipona scutellaris
1.14.14.127
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis
Melipona scutellaris
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.202
cytochrome P450
-
Melipona scutellaris
1.14.13.202
NADPH
-
Melipona scutellaris
1.14.14.127
cytochrome P450
-
Melipona scutellaris
1.14.14.127
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Melipona scutellaris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
Melipona scutellaris
-
juvenile hormone III + NADP+ + H2O
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Melipona scutellaris
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.13.202
adult
-
Melipona scutellaris
-
1.14.13.202
corpus allatum
gene mfe is exclusively expressed in the corpora allata
Melipona scutellaris
-
1.14.13.202
larva
-
Melipona scutellaris
-
1.14.13.202
additional information
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
Melipona scutellaris
-
1.14.13.202
pupa
-
Melipona scutellaris
-
1.14.14.127
adult
-
Melipona scutellaris
-
1.14.14.127
corpus allatum
gene mfe is exclusively expressed in the corpora allata
Melipona scutellaris
-
1.14.14.127
larva
-
Melipona scutellaris
-
1.14.14.127
additional information
gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ
Melipona scutellaris
-
1.14.14.127
pupa
-
Melipona scutellaris
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.13.202
methyl (2E,6E)-farnesoate + NADPH + H+ + O2
-
745508
Melipona scutellaris
juvenile hormone III + NADP+ + H2O
-
-
-
?
1.14.14.127
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
745508
Melipona scutellaris
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.13.202
metabolism
the enzyme catalyzes the final step in juvenile hormone, JH, biosynthesis
Melipona scutellaris
1.14.14.127
metabolism
the enzyme catalyzes the final step in juvenile hormone, JH, biosynthesis
Melipona scutellaris
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.13.202
metabolism
the enzyme catalyzes the final step in juvenile hormone, JH, biosynthesis
Melipona scutellaris
1.14.14.127
metabolism
the enzyme catalyzes the final step in juvenile hormone, JH, biosynthesis
Melipona scutellaris