BRENDA - Enzyme Database

Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea

Shimomura, O.; Johnson, F.H.; Saiga, Y.; J. Cell. Comp. Physiol. 59, 223-239 (1962)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.13.12.24
protein is relatively stable in 0.01 M Na2EDTA
Aequorea aequorea
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.12.24
Barbiturate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
Cyanate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
EDTA
-
Aequorea aequorea
1.13.12.24
glutathione
10 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
H2O2
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
malonate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
NaAsO2
20 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
nitrite
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
thiocyanate
50 mM, complete loss of activity
Aequorea aequorea
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.24
Ca2+
required for luminescence reaction
Aequorea aequorea
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
1.13.12.24
benzyl alcohol
50 mM, 10% increase in activity
Aequorea aequorea
1.13.12.24
n-amyl alcohol
50 mM, 7% increase in activity
Aequorea aequorea
1.13.12.24
n-Butanol
50 mM, 8% increase in activity
Aequorea aequorea
1.13.12.24
n-heptanol
50 mM, 30% increase in activity
Aequorea aequorea
1.13.12.24
n-hexanol
50 mM, 10% increase in activity
Aequorea aequorea
1.13.12.24
n-octanol
50 mM, 18% increase in activity
Aequorea aequorea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.12.24
Aequorea aequorea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.13.12.24
-
Aequorea aequorea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.12.24
5.1
8.3
-
Aequorea aequorea
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.13.12.24
4
-
unstable below
Aequorea aequorea
General Stability (protein specific)
EC Number
General Stability
Organism
1.13.12.24
protein is relatively stable in 0.01 M Na2EDTA
Aequorea aequorea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.12.24
Barbiturate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
Cyanate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
EDTA
-
Aequorea aequorea
1.13.12.24
glutathione
10 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
H2O2
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
malonate
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
NaAsO2
20 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
nitrite
50 mM, complete loss of activity
Aequorea aequorea
1.13.12.24
thiocyanate
50 mM, complete loss of activity
Aequorea aequorea
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.12.24
Ca2+
required for luminescence reaction
Aequorea aequorea
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
1.13.12.24
benzyl alcohol
50 mM, 10% increase in activity
Aequorea aequorea
1.13.12.24
n-amyl alcohol
50 mM, 7% increase in activity
Aequorea aequorea
1.13.12.24
n-Butanol
50 mM, 8% increase in activity
Aequorea aequorea
1.13.12.24
n-heptanol
50 mM, 30% increase in activity
Aequorea aequorea
1.13.12.24
n-hexanol
50 mM, 10% increase in activity
Aequorea aequorea
1.13.12.24
n-octanol
50 mM, 18% increase in activity
Aequorea aequorea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.12.24
-
Aequorea aequorea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.12.24
5.1
8.3
-
Aequorea aequorea
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.13.12.24
4
-
unstable below
Aequorea aequorea