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Literature summary extracted from

  • Shimomura, O.; Johnson, F.H.; Saiga, Y.
    Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea (1962), J. Cell. Comp. Physiol., 59, 223-239 .
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.13.12.24 protein is relatively stable in 0.01 M Na2EDTA Aequorea forskalea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.24 Barbiturate 50 mM, complete loss of activity Aequorea forskalea
1.13.12.24 Cyanate 50 mM, complete loss of activity Aequorea forskalea
1.13.12.24 EDTA
-
Aequorea forskalea
1.13.12.24 glutathione 10 mM, complete loss of activity Aequorea forskalea
1.13.12.24 H2O2 50 mM, complete loss of activity Aequorea forskalea
1.13.12.24 malonate 50 mM, complete loss of activity Aequorea forskalea
1.13.12.24 NaAsO2 20 mM, complete loss of activity Aequorea forskalea
1.13.12.24 nitrite 50 mM, complete loss of activity Aequorea forskalea
1.13.12.24 thiocyanate 50 mM, complete loss of activity Aequorea forskalea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.24 Ca2+ required for luminescence reaction Aequorea forskalea

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.12.24 benzyl alcohol 50 mM, 10% increase in activity Aequorea forskalea
1.13.12.24 n-amyl alcohol 50 mM, 7% increase in activity Aequorea forskalea
1.13.12.24 n-Butanol 50 mM, 8% increase in activity Aequorea forskalea
1.13.12.24 n-heptanol 50 mM, 30% increase in activity Aequorea forskalea
1.13.12.24 n-hexanol 50 mM, 10% increase in activity Aequorea forskalea
1.13.12.24 n-octanol 50 mM, 18% increase in activity Aequorea forskalea

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.24 Aequorea forskalea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.24
-
Aequorea forskalea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.24 5.1 8.3
-
Aequorea forskalea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.12.24 4
-
unstable below Aequorea forskalea