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Literature summary extracted from

  • Lee, H.; Kim, B.; Ahn, J.
    Production of bioactive hydroxyflavones by using monooxygenase from Saccharothrix espanaensis (2014), J. Biotechnol., 176, 11-17 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.14.1 synthesis coexpression of P450 reductase with Sam5 in Escherichia coli enhances hydroxylation by approximately from 34 to 50%, depending on the flavonoid used. Optimization of the production of bioactive flavonoids, 8-hydroxyluteolin and 3'-hydroxydaidzein, in this system gives 88 mg/l of 8-hydroxyluteolin and 75 mg/l of 3'-hydroxydaidzein Saccharothrix espanaensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.1 expression in Escherichia coli Saccharothrix espanaensis

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.1 Saccharothrix espanaensis Q2EYY8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.1 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis 3',8-dihydroxyapigenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.1 daidzein + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis 3'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.1 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis 6-hydroxyeriodictyol + 8-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.1 genistein + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis 3',8-dihydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.1 luteolin + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis 3',8-dihydroxyapigenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.1 additional information isoform Sam5 hydroxylates isoflavones, flavanones, and flavones but does not produce any detectable hydroxylated product with flavonols. Naringenin is the most preferred substrate, followed by eriodictyol, genistein,apigenin, luteolin, and daidzein Saccharothrix espanaensis ?
-
 ?
1.14.14.1 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Saccharothrix espanaensis eriodictyol + 6-hydroxyeriodictyol + 8-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.1 Sam5
-
 Saccharothrix espanaensis