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Literature summary extracted from

  • Wu, H.; Cheng, X.; Zhu, Y.; Zeng, W.; Chen, G.; Liang, Z.
    Purification and characterization of a cellulase-free, thermostable endo-xylanase from Streptomyces griseorubens LH-3 and its use in biobleaching on eucalyptus kraft pulp (2018), J. Biosci. Bioeng., 125, 46-51 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.8 degradation application of enzyme for biobleaching of Eucalyptus kraft pulp, the xylanase increases the brightness of the pulp by 14.5% and reduces the kappa number by 24.5% Streptomyces griseorubens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8
-
 Streptomyces griseorubens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Al3+ 5 mM, 25% of initial activity Streptomyces griseorubens
3.2.1.8 Ca2+ 5 mM, 76% of initial activity Streptomyces griseorubens
3.2.1.8 Cu2+ 5 mM, 73% of initial activity Streptomyces griseorubens
3.2.1.8 Hg2+ 5 mM, 8% of initial activity Streptomyces griseorubens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 birchwood xylan Km value 1.44 mg/ml, pH 5.0, 50°C Streptomyces griseorubens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.8 Fe2+ 5 mM, 113% of initial activity Streptomyces griseorubens
3.2.1.8 Mg2+ 5 mM, 110% of initial activity Streptomyces griseorubens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 45500
-
 gel filtration Streptomyces griseorubens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Streptomyces griseorubens
-
-
-
3.2.1.8 Streptomyces griseorubens LH-3
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 434
-
 pH 5.0, 50°C Streptomyces griseorubens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 birchwood xylan + H2O
-
 Streptomyces griseorubens ?
-
 ?
3.2.1.8 birchwood xylan + H2O
-
 Streptomyces griseorubens LH-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 monomer 1 * 45500, SDS-PAGE Streptomyces griseorubens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 60
-
-
 Streptomyces griseorubens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 1 h, 60% residual activity Streptomyces griseorubens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5
-
-
 Streptomyces griseorubens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.8 4 8 more than 80% of maxiumum activity Streptomyces griseorubens