BRENDA - Enzyme Database show

Myxococcus xanthus low-molecular-weight protein tyrosine phosphatase homolog, ArsA, possesses arsenate reductase activity

Mori, Y.; Kimura, Y.; J. Biosci. Bioeng. 118, 10-13 (2014)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
dithiothreitol
enzyme activity is increased about 1.5fold by the addition of 2 mM dithiothreitol
Myxococcus xanthus
1.20.4.1
additional information
1 mM EDTA has no apparent effect on enzyme activity
Myxococcus xanthus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
4.3
-
arsenate
at pH 9.0, temperature not specified in the publication
Myxococcus xanthus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.4.1
additional information
the enzyme is not stimulated by 1 mM MgCl2, MnCl2, CuCl2, CaCl2, ZnCl2, NiCl2, FeCl2 or FeCl3
Myxococcus xanthus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenite + acceptor
Myxococcus xanthus
high activity
arsenate + reduced acceptor
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.20.4.1
Myxococcus xanthus
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + glutaredoxin
-
745405
Myxococcus xanthus
arsenite + glutaredoxin disulfide + H2O
-
-
-
?
1.20.4.1
arsenate + glutathione
-
745405
Myxococcus xanthus
arsenite + glutathione disulfide + H2O
-
-
-
?
1.20.4.1
arsenite + acceptor
high activity
745405
Myxococcus xanthus
arsenate + reduced acceptor
-
-
-
?
1.20.4.1
additional information
the enzyme exhibits weak phosphatase activity toward 4-nitrophenyl phosphate
745405
Myxococcus xanthus
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.20.4.1
?
x * 16000, SDS-PAGE
Myxococcus xanthus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.4.1
50
-
-
Myxococcus xanthus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
9
-
-
Myxococcus xanthus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.20.4.1
dithiothreitol
enzyme activity is increased about 1.5fold by the addition of 2 mM dithiothreitol
Myxococcus xanthus
1.20.4.1
additional information
1 mM EDTA has no apparent effect on enzyme activity
Myxococcus xanthus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.20.4.1
4.3
-
arsenate
at pH 9.0, temperature not specified in the publication
Myxococcus xanthus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.20.4.1
additional information
the enzyme is not stimulated by 1 mM MgCl2, MnCl2, CuCl2, CaCl2, ZnCl2, NiCl2, FeCl2 or FeCl3
Myxococcus xanthus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.20.4.1
arsenite + acceptor
Myxococcus xanthus
high activity
arsenate + reduced acceptor
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.20.4.1
arsenate + glutaredoxin
-
745405
Myxococcus xanthus
arsenite + glutaredoxin disulfide + H2O
-
-
-
?
1.20.4.1
arsenate + glutathione
-
745405
Myxococcus xanthus
arsenite + glutathione disulfide + H2O
-
-
-
?
1.20.4.1
arsenite + acceptor
high activity
745405
Myxococcus xanthus
arsenate + reduced acceptor
-
-
-
?
1.20.4.1
additional information
the enzyme exhibits weak phosphatase activity toward 4-nitrophenyl phosphate
745405
Myxococcus xanthus
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.20.4.1
?
x * 16000, SDS-PAGE
Myxococcus xanthus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.20.4.1
50
-
-
Myxococcus xanthus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.20.4.1
9
-
-
Myxococcus xanthus