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Literature summary extracted from

  • Boyarshin, K.S.; Priss, A.E.; Rayevskiy, A.V.; Ilchenko, M.M.; Dubey, I.Y.; Kriklivyi, I.A.; Yaremchuk, A.D.; Tukalo, M.A.
    A new mechanism of post-transfer editing by aminoacyl-tRNA synthetases catalysis of hydrolytic reaction by bacterial-type prolyl-tRNA synthetase (2017), J. Biomol. Struct. Dyn., 35, 669-682 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli BL21(DE3)Star cells Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.15 G331A the mutant shows 6.7fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 G334A the mutant shows 2.2fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 H366A the mutant shows 4.4fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 I263A the mutant shows 2.4fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 I278A the mutant shows 12.8fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 I333A the mutant shows 54.7fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 K279A the mutant shows 2006.3fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 S280A the mutant shows 2fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 S332A the mutant shows 1.1fold increased activity compared to the wild type enzyme Enterococcus faecalis
6.1.1.15 T257A the mutant shows 1.1fold increased activity compared to the wild type enzyme Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Enterococcus faecalis
-
AMP + diphosphate + L-prolyl-tRNAPro
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Enterococcus faecalis Q831W7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 DEAE-Sepharose column chromatography and Ni-NTA agarose column chromatography Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + L-tRNAAla
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAAla
-
?
6.1.1.15 ATP + L-proline + L-tRNAPro
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAPro
-
?
6.1.1.15 ATP + L-proline + L-tRNAProAla
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAProAla
-
?
6.1.1.15 ATP + L-proline + tRNAPro
-
Enterococcus faecalis AMP + diphosphate + L-prolyl-tRNAPro
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
Enterococcus faecalis
6.1.1.15 ProRS
-
Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
Enterococcus faecalis