EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.53 | 0.0011 | - |
all-trans-retinol | pH 7.4, 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.53 | Homo sapiens | Q4G0S4 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.19.53 | skin | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.53 | all-trans-retinol + 2 reduced adrenodoxin + 2 H+ + O2 | - |
Homo sapiens | all-trans-3,4-didehydroretinol + 2 oxidized adrenodoxin + 2 H2O | - |
? | |
1.14.19.53 | additional information | kinetic isotope effects of 1.52.3 (on kcat/Km) are observed with 3,3-, 4,4-, and 3,3,4,4-deuterated retinol. Deuteration at C-4 produces a 4fold increase in 3-hydroxylation due to metabolic switching, with no observable effect on 4-hydroxylation. Deuteration at C-3 produces a strong kinetic isotope effect for 3-hydroxylation but not 4-hydroxylation | Homo sapiens | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.53 | 0.068 | - |
all-trans-retinol | pH 7.4, 37°C | Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.53 | physiological function | the most likely catalytic mechanism begins with abstraction of a hydrogen atom from C-4 (or possibly C-3) initiating the desaturation pathway, followed by a sequential abstraction of a hydrogen atom or proton-coupled electron transfer. Adrenodoxin reduction and hydrogen abstraction both contribute to rate limitation | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.53 | 61 | - |
all-trans-retinol | pH 7.4, 37°C | Homo sapiens |