BRENDA - Enzyme Database

BciD is a radical S-adenosyl-L-methionine (SAM) enzyme that completes bacteriochlorophyllide e biosynthesis by oxidizing a methyl group into a formyl group at C-7

Thweatt, J.; Ferlez, B.; Golbeck, J.; Bryant, D.; J. Biol. Chem. 292, 1361-1373 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.17.98.2
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum limnaeum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.17.98.2
Chlorobaculum limnaeum
N0DKX5
-
-
1.17.98.2
Chlorobaculum limnaeum DSMZ 1677
N0DKX5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.17.98.2
Ni-NTA column chromatography, and Superdex 200 gel filtration
Chlorobaculum limnaeum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.17.98.2
?
x * 44000, SDS-PAGE
Chlorobaculum limnaeum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.17.98.2
S-adenosyl-L-methionine
-
Chlorobaculum limnaeum
1.17.98.2
[4Fe-4S]-center
-
Chlorobaculum limnaeum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.98.2
expressed in Escherichia coli BL21(DE3) cells
Chlorobaculum limnaeum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.17.98.2
S-adenosyl-L-methionine
-
Chlorobaculum limnaeum
1.17.98.2
[4Fe-4S]-center
-
Chlorobaculum limnaeum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
Chlorobaculum limnaeum DSMZ 1677
-
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.17.98.2
Ni-NTA column chromatography, and Superdex 200 gel filtration
Chlorobaculum limnaeum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a 7-(hydroxymethyl)bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(dihydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a 7-(hydroxymethyl)bacteriochlorophyllide c + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide c + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide e + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
1.17.98.2
S-adenosyl-L-methionine + a bacteriochlorophyllide d + H2O
-
745366
Chlorobaculum limnaeum DSMZ 1677
a bacteriochlorophyllide f + 5'-deoxyadenosine + L-methionine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.17.98.2
?
x * 44000, SDS-PAGE
Chlorobaculum limnaeum