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Literature summary extracted from

  • Wang, X.; Liu, L.
    Crystal structure and catalytic mechanism of 7-hydroxymethyl chlorophyll a reductase (2016), J. Biol. Chem., 291, 13349-13359 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.2 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.7.2 sitting drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5, 25% (w/v) polyethylene glycol 3350, 3% (v/v) ethylene glycol Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.17.7.2 D237A inactive Arabidopsis thaliana
1.17.7.2 E262A the mutant loses its catalytic activity, probably for its impaired FAD binding capacity Arabidopsis thaliana
1.17.7.2 H417A the mutant formes inclusion bodies during expression, and hence its activity is not measureable Arabidopsis thaliana
1.17.7.2 H417N inactive Arabidopsis thaliana
1.17.7.2 H417Y inactive Arabidopsis thaliana
1.17.7.2 Q332A the mutant aggregates and its activity is not measureable Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.7.2 195000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.2 71-hydroxychlorophyll a + reduced ferredoxin + H+ Arabidopsis thaliana
-
 chlorophyll a + oxidized ferredoxin + H2O
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.2 Arabidopsis thaliana Q8GS60
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.7.2 Ni-NTA column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.2 71-hydroxychlorophyll a + reduced ferredoxin + H+
-
 Arabidopsis thaliana chlorophyll a + oxidized ferredoxin + H2O
-
 ir

Subunits

EC Number Subunits Comment Organism
1.17.7.2 trimer 3 * 49000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.17.7.2 HCAR
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.2 FAD
-
 Arabidopsis thaliana
1.17.7.2 [4Fe-4S]-center
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.17.7.2 metabolism the enzyme is required for the degradation of light-harvesting complexes and is necessary for efficient photosynthesis by balancing the chlorophyll a/b ratio Arabidopsis thaliana