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Literature summary extracted from

  • Di Nardo, G.; Breitner, M.; Bandino, A.; Ghosh, D.; Jennings, G.K.; Hackett, J.C.; Gilardi, G.
    Evidence for an elevated aspartate pK(a) in the active site of human aromatase (2015), J. Biol. Chem., 290, 1186-1196 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.14 D309N mutant retains its ability to bind to androstenedione across the entire pH range studied Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.14 anastrozole azole inhibitor, binding is independent of pH Homo sapiens
1.14.14.14 exemestane steroidal inhibitor, binding is dependent of pH Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.14 additional information
-
 androst-4-ene-3,17-dione the Km values increase 1.5fold from pH 6.5 to 7.4similar to the Kd values, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.14 Homo sapiens P11511
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.14 androst-4-ene-3,17-dione + 3 O2 + 3 reduced flavoproteins
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 Homo sapiens estrone + formate + 4 H2O + 3 oxidized flavoproteins
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.14 CYP19A1
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.14.14 additional information
-
 androstenedione binding is pH-dependent. The apparent pKa for Asp309 is 8.2, and the residue is protonated at physiological pH. A D309N mutant binds androstenedione across the entire pH range studied Homo sapiens