BRENDA - Enzyme Database show

Dual functions of the trans-2-enoyl-CoA reductase TER in the sphingosine 1-phosphate metabolic pathway and in fatty acid elongation

Wakashima, T.; Abe, K.; Kihara, A.; J. Biol. Chem. 289, 24736-24748 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.3.1.93
gene TECR
Rattus norvegicus
1.3.1.93
gene TECR, cloning from liver cDNA, Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells
Homo sapiens
1.3.1.93
gene TSC13, recombinant expression as N-terminal FLAG3-tagged enzyme from pAKNF313 and pAKNF315, or as N-terminal HA2-tagged enzyme
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.3.1.93
additional information
HeLa cells are transfected with control siRNA or TER si RNA, siRNA-generated enzyme knockout mutant. Knockdown of TER in HeLa cells causes decreased sphingosine 1-phosphate metabolism in vitro and a reduction in the dihydrosphingosine metabolism
Homo sapiens
1.3.1.93
additional information
YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.93
endoplasmic reticulum
-
Homo sapiens
5783
-
1.3.1.93
endoplasmic reticulum
-
Rattus norvegicus
5783
-
1.3.1.93
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Homo sapiens
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Rattus norvegicus
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Homo sapiens
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Rattus norvegicus
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
palmitoyl-CoA + NADP+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.3.1.93
Homo sapiens
Q9NZ01
-
-
1.3.1.93
Rattus norvegicus
Q64232
-
-
1.3.1.93
Saccharomyces cerevisiae
-
-
-
1.3.1.93
Saccharomyces cerevisiae BY4741
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.93
HeLa cell
-
Homo sapiens
-
1.3.1.93
Hep-G2 cell
-
Homo sapiens
-
1.3.1.93
hepatocyte
-
Homo sapiens
-
1.3.1.93
IEC-6 cell
-
Rattus norvegicus
-
1.3.1.93
intestinal epithelial cell
-
Rattus norvegicus
-
1.3.1.93
neuronal cell
-
Rattus norvegicus
-
1.3.1.93
PC-12 cell
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Homo sapiens
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Rattus norvegicus
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Homo sapiens
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Rattus norvegicus
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
palmitoyl-CoA + NADP+
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.93
NADPH
-
Homo sapiens
1.3.1.93
NADPH
-
Rattus norvegicus
1.3.1.93
NADPH
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.3.1.93
gene TECR
Rattus norvegicus
1.3.1.93
gene TECR, cloning from liver cDNA, Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells
Homo sapiens
1.3.1.93
gene TSC13, recombinant expression as N-terminal FLAG3-tagged enzyme from pAKNF313 and pAKNF315, or as N-terminal HA2-tagged enzyme
Saccharomyces cerevisiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.3.1.93
NADPH
-
Homo sapiens
1.3.1.93
NADPH
-
Rattus norvegicus
1.3.1.93
NADPH
-
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.3.1.93
additional information
HeLa cells are transfected with control siRNA or TER si RNA, siRNA-generated enzyme knockout mutant. Knockdown of TER in HeLa cells causes decreased sphingosine 1-phosphate metabolism in vitro and a reduction in the dihydrosphingosine metabolism
Homo sapiens
1.3.1.93
additional information
YRF50 cells (BY4741,pTSC13::KanMX4-tTA-ptetO7) are constructed by replacing the promoter of the TSC13 gene (pTSC13) with tetO7 promoter (ptetO7) using the KanMX4-tTA-ptetO7 cassette from the pCM225 plasmid. Strains ABY83 and ABY80 cells are constructed by deletion of the TSC13 gene in BY4741 cells bearing the pTW6 or pAB119 plasmid, respectively, using a tsc13DELTA::LEU2 fragment by homologous recombination. Generation of Saccharomyces cerevisiae Tsc13-lowered cells
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.3.1.93
endoplasmic reticulum
-
Homo sapiens
5783
-
1.3.1.93
endoplasmic reticulum
-
Rattus norvegicus
5783
-
1.3.1.93
endoplasmic reticulum
-
Saccharomyces cerevisiae
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Homo sapiens
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Rattus norvegicus
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
a very-long-chain acyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Homo sapiens
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Rattus norvegicus
-
palmitoyl-CoA + NADP+
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
Saccharomyces cerevisiae BY4741
-
palmitoyl-CoA + NADP+
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.3.1.93
HeLa cell
-
Homo sapiens
-
1.3.1.93
Hep-G2 cell
-
Homo sapiens
-
1.3.1.93
hepatocyte
-
Homo sapiens
-
1.3.1.93
IEC-6 cell
-
Rattus norvegicus
-
1.3.1.93
intestinal epithelial cell
-
Rattus norvegicus
-
1.3.1.93
neuronal cell
-
Rattus norvegicus
-
1.3.1.93
PC-12 cell
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Homo sapiens
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Rattus norvegicus
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
a very-long-chain trans-2,3-dehydroacyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
a very-long-chain acyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Homo sapiens
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Rattus norvegicus
palmitoyl-CoA + NADP+
-
-
-
?
1.3.1.93
trans-2-hexadecenoyl-CoA + NADPH + H
-
745307
Saccharomyces cerevisiae BY4741
palmitoyl-CoA + NADP+
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.3.1.93
malfunction
in membrane fractions prepared from TER siRNA-treated HeLa cells, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA is largely impaired, and only a small amount of palmitoyl-CoA is produced. Instead, trans-2-hexadecenoyl-CoA is the main product, and C14:0-CoA is also detected
Homo sapiens
1.3.1.93
malfunction
ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway, lethality of VLCFA-deficient mutations
Saccharomyces cerevisiae
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview. Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway
Homo sapiens
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Rattus norvegicus
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Saccharomyces cerevisiae
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Homo sapiens
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Rattus norvegicus
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Saccharomyces cerevisiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.3.1.93
malfunction
in membrane fractions prepared from TER siRNA-treated HeLa cells, the conversion of trans-2-hexadecenoyl-CoA to palmitoyl-CoA is largely impaired, and only a small amount of palmitoyl-CoA is produced. Instead, trans-2-hexadecenoyl-CoA is the main product, and C14:0-CoA is also detected
Homo sapiens
1.3.1.93
malfunction
ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway, lethality of VLCFA-deficient mutations
Saccharomyces cerevisiae
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview. Ectopic expression of human trans-2-enoyl-CoA reductase TER in Saccharomyces cerevisiae TER homologue Tsc13-lowered cells causes recovery in the deficient sphingosine 1-phosphate metabolic pathway
Homo sapiens
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Rattus norvegicus
1.3.1.93
metabolism
TER is involved sphingosine degradation within sphingolipids in the S1P metabolic pathway. trans-2-enoyl-CoA reductase TER catalyzes the saturation step of the sphingosine 1-phosphate (S1P) metabolic pathway. The pathways of sphingolipid degradation and synthesis, overview
Saccharomyces cerevisiae
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Homo sapiens
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Rattus norvegicus
1.3.1.93
physiological function
the trans-2-enoyl-CoA reductase, TER, functions in very long-chain fatty acid (VLCFA) synthesis and is involved in the fatty acid elongation cycle, where palmitic acid synthesized by fatty acid synthase or fatty acids taken from foods are elongated to very long-chain fatty acids (VLCFAs) with carbon chain lengths greater than 20
Saccharomyces cerevisiae